Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Authors
Abstract:
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated distinct RAPD profiles. The results indicated genotypic diversity and heterogeneity among MG isolated from field, which can be used for epidemiological studies and for differentiation between vaccine strain, and field isolates.
similar resources
random amplified polymorphic dna (rapd) fingerprinting of mycoplasma gallisepticum isolates from chickens
ten field isolates of mycoplasma gallisepticum (mg) from different geographical areas of iran were analyzed by random amplification of polymorphic dna (rapd). rapd analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. mg isolates compared with reference strains (s6 and mg ss) and vaccine strain (ts-11) and demonstrated d...
full textRandom Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
full textRandom amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey
Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT stra...
full textReproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method.
Much interest has recently arisen in methods for DNA fingerprinting based on the polymerase chain reaction (PCR). Among these, the Random Amplified Polymorphic DNA (RAPD) method, developed by Williams etal. (1), is currently receiving particular attention (2) because of its extreme simplicity and requirement for minimal amounts of genomic DNA. The basic strategy involves the PCR amplification o...
full textMolecular epidemiologic investigations of Mycoplasma gallisepticum conjunctivitis in songbirds by random amplified polymorphic DNA analyses.
An ongoing outbreak of conjunctivitis in free-ranging house finches (Carpodacus mexicanus) began in 1994 in the eastern United States. Bacterial organisms identified as Mycoplasma gallisepticum (MG) were isolated from lesions of infected birds. MG was also isolated from a blue jay (Cyanocitta cristata) that contracted conjunctivitis after being housed in a cage previously occupied by house finc...
full textMolecular Epidemiologic Investigations of Mycoplasma gallisepticum Conjunctivitis in Songbirds by Random Amplified Polymorphic DNA Analyses
Mycoplasma gallisepticum (MG), a wellknown cause of diseases of domesticated chickens and turkeys worldwide, most notably causes chronic respiratory disease in chickens and infectious sinusitis in turkeys (1). Although MG is a known pathogen of other gallinaceous birds and has been isolated from ducks and geese, it has not been considered a natural pathogen of wild birds, including songbirds (1...
full textMy Resources
Journal title
volume 61 issue 2
pages 67-
publication date 2006-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023