Protective Effect of Wheat Germ Oil on Sex Hormones Disorders and Uterine Tissue Disorders Morphology Following Treatment with Carbamazepine in Epileptic Female Mice with Pentylenetetrazol

  Background & Objective: Epilepsy is one of the common neurological diseases and carbamazepine is an antiepileptic drug that causes oxidative stress, endocrine and the reproductive system long term disorders. The aim of this study is to evaluate the effect of wheat germ oil, as a rich source of antioxidants on sex hormones and uterine tissue in epileptic mice treated with carbamazepine. Materials & Methods: In this study 48 adult female mice were randomly divided into 6 groups (n=8). Including: 1- control. 2- received pentylenetetrazol at dose of 40 (seizure control). 3- received pentylenetetrazol + carbamazepine at dose of 30. 4- received pentylenetetrazol + carbamazepine at dose of 180. 5- received pentylenetetrazol + carbamazepineat dose of 30 + wheat germ ‌oil receiver at dose of 500. 6- received pentylenetetrazol + carbamazepine at dose of 180 + wheat‌ germ ‌oil at dose of 500.(units of doses are as grams/kilogram of body weight). At the end of the treatment period, ELISA method was used to measure estrogen, progesterone and testosterone levels . In addition, histological studies were performed on the uterus. Data were analyzed using ANOVA and Tukey's test in SPSS20 software.   Results: Carbamazepine levels of estrogen, progesterone, testosterone, and except of myometrium, the uterine thickness and average uterine glands decreased significantly. In the group with wheat germ oil  + carbamazepine (30, 180), levels of mentioned factors changed near-normal significantly.   Conclusion: Results showed that the wheat germ oil improves reproductive system performance and reduces the complication of carbamazepine.