Prolonged Laboratory Maintenance of Toxoplasma gondii Tachyzoites in Co-cultivation with the Bovine Theileria annulata-Infected Lymphoblastoids
author
Abstract:
Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been maintained up to 260 sub-cultures to attenuate the Theileria annulata, were evaluated for their suitability to the cultivation of Toxoplasma gondii tachyzoites. This cultivation process was carried out continuously for up to 10 passages, and after each 5 sub-culture, 0.1 ml of culture suspension (1×106 tachyzoites) was inoculated to each BALB/c mouse. Results: It was observed that the tachyzoites have attacked the lympho-blastoids, multiplied inside them, and many fresh tachyzoites with typical shape and gliding movement were present in the culture suspension. In all processes of cultivation, the pathogenesis of parasites remained stable, and they were able to proliferate in mice and eventually lead to the death of the animals. Conclusions: We describe here a new protocol for prolonged maintenance of tachyzoites of Toxoplasma gondii, which is more efficient (both in terms of yield and cost (it does not need fetal calf serum)) than other traditional methods for maintenance of the parasite.
similar resources
Acidocalcisomes in Toxoplasma gondii tachyzoites.
Toxoplasma gondii tachyzoites were loaded with the fluorescent indicator fura 2 to investigate the transport mechanisms involved in maintaining their intracellular Ca2+ homoeostasis. The mitochondrial ATPase inhibitor oligomycin and the endoplasmic-reticulum Ca(2+)-ATPase inhibitor thapsigargin increased the intracellular Ca2+ concentration ([Ca2+]i), thus indicating the requirement for ATP and...
full textImmunogenicity of Gamma-Irradiated Toxoplasma gondii Tachyzoites in Mice
The Immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of Toxoplasma gondii (RH strain) was investigated. Immunized mice survived challenge infection and displayed increased interferon gamma (INF-γ ) production. On the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to Concanavalin A (Con A). No tachyz...
full textCharacterization of HSP90 isoforms in transformed bovine leukocytes infected with Theileria annulata
HSP90 chaperones are essential regulators of cellular function, as they ensure the appropriate conformation of multiple key client proteins. Four HSP90 isoforms were identified in the protozoan parasite Theileria annulata. Partial characterization was undertaken for three and localization confirmed for cytoplasmic (TA12105), endoplasmic reticulum (TA06470), and apicoplast (TA10720) forms. ATPas...
full textThe Therapeutic Value of Oxyteracycline Hydrochloride (Terramycin) in Cattle Infected Experimentally with Theileria annulata
full text
Immune Responses of Mice Intraduodenally Infected with Toxoplasma gondii KI-1 Tachyzoites
Toxoplasma gondii Korean isolate (KI-1) tachyzoites were inoculated intraduodenally to BALB/c mice using a silicon tube, and the course of infection and immune responses of mice were studied. Whereas control mice, that were infected intraperitoneally, died within day 7 post-infection (PI), the intraduodenally infected mice survived until day 9 PI (infection with 1 × 10(5) tachyzoites) or day 11...
full textBiochemical, Hematological studies in cattle naturally infected with Theileria annulata
The aim of the present study dealt with biochemical and hematological changes in cattle which presented to the Azad clinic of veterinary school in Urmia during the period between Dec 2008 to January 2012. A total of 985 cattle with positive theileriosis were examined. Data were collected on hematological conditions of the cattle. Ages of these slaughtered buffaloes ranged from 6 to 9 years, (79...
full textMy Resources
Journal title
volume 8 issue 1
pages 27- 34
publication date 2021-02
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023