Production of bacteriocin by a novel Bacillus sp. strain RF 140, an intestinal bacterium of Caspian Frisian Roach (Rutilus frisii kutum)

Authors

  • Gh. Shah-Hosseini Department of Nuclear Agriculture Research, School of Agricultural, Medical and Industrial, Nuclear Science Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran
  • M. Ghanbari Department of Fisheries, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Mazandaran, Iran
  • M. Rezaei Department of Fisheries, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Mazandaran, Iran
  • M. Soltani Department of Health Hygiene and Aquatic Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:

Bacteriocins are proteinaceous antibacterial compounds that exhibit bactericidal activity against species closely related to the producer strain. The aim of this research was to investigate the production of bacteriocin by Bacillus spp. isolated from intestinal bacterial flora of the Caspian Frisian Roach (Rutilus frisii kutum). A bacteriocin produced by the bacterium Bacillus cereus strain RF 140 was identified. The antimicrobial activity started at the exponential growth phase and maximum activity was at the stationary growth phase. A crude bacteriocin obtained from culture supernatant fluid was inhibitory to indicator strains,including Listeria monocytogenes, Clostridium perfringens, and several species of Bacillus. Bacteriocin was stable at 80°C, but the activity decreased and was lost when the temperature reached 100 and 121°C, respectively. It was resistant to the proteolytic action of papain, catalase and amylase, but sensitive to proteinase K, pronase E and trypsin. Maximum bacteriocin activity was observed in the pH 4-9. This study indicates the importance of the bacteriocin produced by B. cereus  strain RF 140 against food-borne pathogenic microorganisms such as L. monocytogenes and C. perfringens , and presents a potential for use as a biopreservative in food.    

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Journal title

volume 10  issue 3

pages  267- 272

publication date 2009-09-30

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