Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention

Authors

  • Ahmad Reza Bahrami Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Asieh Heirani-Tabasi Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Hamid Reza Bidkhori Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Hojjat Naderi-Meshkin Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Mahmood Raisolmohaddesin Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Mahmoud Hosseini Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Maliheh Hasanzadeh Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Maryam M. Matin Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Mina Shahryari Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.
  • Naghmeh Ahmadiankia Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Nasser Sanjar Moussavi Department of Surgery, Faculty of Medicine, Islamic Azad University-Mashhad Branch, Iran.
Abstract:

Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs in vivo, 2 weeks after intravenous (IV), local infusion towards subcutaneously implanted chitosan-glycerophophate-hydroxyethyl cellulose (CH-GP-HEC) injectable hydrogels releasing SDF1 in dorsum of Wistar Rats. Presence of human ASCs in the CH-GP-HEC injectable, spleen, and lung were analyzed histologically by fluorescent microscope, and also quantified by PCR for human specific CXCR4 gene, 2 weeks after transplantation in recipients' Rats. Results showed that short-term (24 hours) pretreatment to ASCs with the hypoxia mimicking agents up-regulate the CXCR4, increase in vitro migration capacity toward 100ng/ml SDF-1 (P

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

pretreatment of mesenchymal stem cells and stromal-derived factor-1α delivery from chitosan-based injectable hydrogels for better cell guidance and retention

clinical applications of mesenchymal stem cells (mscs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. homing and engraftment capacity of these stem cells depend on the expression of chemokines and their receptors. ex vivo expanded mscs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor bec...

full text

Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells

full text

Biocompatibility evaluation of chitosan-based injectable hydrogels for the culturing mice mesenchymal stem cells in vitro.

A thermosensitive polymer can be held liquid before being injected to deliver living cells or therapeutic agents and formed monolithic gels when injected in vivo. In this study, chitosan-based thermosensitive hydrogels were prepared, characterized and the biocompatibility for culturing mice bone mesenchymal stem cells (BMSC) on 2D films and within 3D hydrogel were investigated. The gelation tem...

full text

Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth.

Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. There...

full text

Mesenchymal stem cell recruitment by stromal derived factor-1-delivery systems based on chitosan/poly(γ-glutamic acid) polyelectrolyte complexes.

Human mesenchymal stem cells (hMSCs) have an enormous potential for tissue engineering and cell-based therapies. With a potential of differentiation into multiple lineages and immune-suppression, these cells play a key role in tissue remodelling and regeneration. Here a method of hMSC recruitment is described, based on the incorporation of a chemokine in Chitosan (Ch)/Poly(γ-glutamic acid) (γ-P...

full text

Injectable alginate-microencapsulated canine adipose tissue-derived mesenchymal stem cells for enhanced viable cell retention

The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 2  issue 2.3

pages  19- 19

publication date 2014-05-01

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023