Preparation of Methotrexate loaded PLGA nanoparticles coated with PVA and Poloxamer188

Authors

  • Arash Goodarzi Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Basil Mujokoro Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Fatemeh Madani Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mahdi Adabi Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mansoureh Hashemi Department of Neuroscience, Functional Neurosurgery Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Masood Khosravani Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:

Objective(s): Nanoparticles offer an attractive platform for drug delivery through a wide variety of the body's physiological barriers. Furthermore, modification of nanoparticle surface with moeites such as Poloxamer188 can enhance their accumulation and localization at disease site. In this work, we investigated the physiochemical effect of a scavenger receptor (SR-BI) interacting moiety coated on the surface of methotrexate (MTX)-loaded PLGA nanoparticles. Methods: Methotrexate-loaded PLGA nanoparticles were prepared by a single step nanoprecipitation technique. The prepared nanoparticles were characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM) for their size and morphology respectively. In vitro drug encapsulation efficiency (EE) and relative drug loading (DL) of nanoparticles were examined by UV-Vis spectrophotometry. Results: The results showed that the mean diameter of nanoparticles and zeta potential increased when more poloxamer188 was added to preparation process.The DL and EE of MTX increased with increase in poloxamer188/PVA ratio. In vitro release of MTX from PLGA nanoparticle was extended by increasing poloxamer188 in preparation process. Conclusions: MTX loaded PLGA nanoparticle modified with PVA and poloxamer 188 with suitable sizes and physiochemical properties can potentially improve drug delivery.  

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Journal title

volume 3  issue 1

pages  19- 24

publication date 2018-01-01

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