Preparation and quality control of radiolabeled streptokinase for Thrombosis imaging

Authors

  • F. Bolourinovin
  • F. Mirazizi
  • H. Nazem
  • H. Sadeghpour
  • J. Garousi
Abstract:

Background: In order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. The radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. Materials and Methods: Streptokinase was successively labeled with [67Ga]-gallium chloride using cyclic DTPA-dianhydride. The conjugation with DTPA was optimized for concentration, time and temperature followed by size exclusion chromatography using G-50 Sephadex. The radiochemical purity of the tracer was checked using HPLC and ITLC methods. The biodistribution studies were performed in normal rats up to 167 h using tissue counting and preliminary SPECT studies up to 2 h. Results: The radiolabeled enzyme was prepared in 60 minutes after incubation at room temperature, with the radiochemical purity of >95% (HPLC) and >99% (ITLC) methods. The radioactivity was accumulated in lung, intestine and liver as shown by scarification and SPECT (Single Photon Emission Computed Tomography) methods. Conclusion: Radiolabeled Streptokinase was prepared in suitable radiochemical purity and its biodistribution is comparable to other radiolabeled proteins. Further studies are required to investigate the imaging properties of the tracer in appropriate animal model. Iran. J. Radiat. Res., 2009 6 (4): 195-200

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

preparation and quality control of radiolabeled streptokinase for thrombosis imaging

background: in order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. the radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. materials and methods: streptokinase was successively labeled with [67ga]-gallium chloride using cyclic dtpa-dianhydride. the conjugation with dtpa was optimized for concentration, time and temperature foll...

full text

Evaluation of radiolabeled streptokinase for thrombosis imaging

  Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodis...

full text

evaluation of radiolabeled streptokinase for thrombosis imaging

introduction: cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. rapid diagnosis of the thrombosis can be an essential step in management of the stroke. methods: in this work a recently developed radiolabeled streptokinase (stp) tracer was evaluated in an animal thrombotic model using spect imaging and biodistribution studies. locally lab...

full text

Evaluation of Radiolabeled Streptokinase for Thrombosis Imaging

Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodistribution studies. Locally lab...

full text

Preparation and quality control of 177Lu-chitosan for radiosynovectomy

Introduction: Rheumatoid arthritis (RA) is the most common autoimmune disease, leading to the destruction of the joints and causing pain, disability, and immobility in the patients. Radiosynovectomy (RSV) has been applied as an effective treatment for the patients with resistant synovitis after failure of long-term pharmacotherapy and intra-articular steroid injection for more than 50 years. Se...

full text

Preparation and Quality Control of 68Ga-Citrate for PET Applications

  Objective(s): In nuclear medicine studies, gallium-68 (68Ga) citrate has been recently known as a suitable infection agent in positron emission tomography (PET). In this study, by applying an in-house produced 68Ge/68Ga generator, a simple technique for the synthesis and quality control of 68Ga-citrate was introduced; followed by preliminary animal studies. Methods: 68GaCl3 eluted from the ge...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 6  issue None

pages  195- 200

publication date 2009-03

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023