Polymorphism of Vascular Endothelial Growth Factor Gene−1154 G/A in Women with Recurrent Miscarriage and IVF Failure; A case–control study

Authors

  • Maryam Eftekhar Professor, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Mojgan Moshrefi Ph.D. Candidate, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Nasrin Ghasemi Professor, Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Saeedeh Soleimanian Ph.D. Candidate , Transplant Research Center Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
  • Soheila Pourmasumi Assistant Professor, Non-Communicable Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract:

Background: Vascular endothelial growth factor A (VEGFA) plays a key role in angiogenesis during human placenta formation and its abnormal expressions have been reported in placental tissues of women with recurrent miscarriage (RM). Objective: The aim of this study was to evaluate the correlation of polymorphism of VEGF1154 G/A gene in RM and In Vitro Fertilization (IVF) failures. Methods: The peripheral blood samples of women with RM, IVF failures and healthy women with live born children, as control group were collected. DNA samples were isolated and VEGF 1154G/A polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism. Single nucleotide polymorphism scanning was done using MnII restriction enzymes for 1154 G/A. Results: The findings of this study showed that the VEGF 1154 A/A and VEGF 1154 G/A mutation frequencies in both RM and IVF failure groups were significantly higher than those in the control group (p=0.005). The homozygous AA mutant genotype frequency in the control group was 0, While in the RM and IVF failure groups it was 30% and 13.9%, respectively (p=0.005). Moreover, the heterozygous AG genotype frequencies were higher in the RM (66.7%) and IVF failure (77.8%) compared to those in the control group (58.3%). Conclusions: It was concluded that VEGF 1154 A/A and VEGF 1154 G/A polymorphisms were associated with both RM and IVF failures. However, their relation with IVF failures was more common than RM in A/G genotype, while in A/A, the RM was higher than IVF failure.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Serum and Peritoneal Fluid Levels of Vascular Endothelial Growth Factor in Women with Endometriosis

full text

Methodological Concerns Regarding Levels of Vascular Endothelial Growth Factor (VEGF) in Serum of Women with Endometriosis

s:865:"We read with interest the manuscript entitled "Serum and peritoneal fluid levels of vascular endothelial growth factor in women with endometriosis" recently published in the International Journal of Fertility and Sterility (1). The authors measured the levels of vascular endothelial growth factor in serum and peritoneal fluid of 179 women, 90 of whom were diagnosed with endometriosis, an...

full text

The Relationship between Vascular Endothelial Growth Factor 1154G/A Polymorphism and Recurrent Implantation Failure

OBJECTIVE The aim of this study was to investigate the relationship between herpesvirus-associated ubiquitin-specific protease (HAUSP A/G, rs1529916), tumor protein p53 (TP53 Arg/Pro, rs1042522), leukemia inhibitory factor (LIF G/T, rs929271), glycoprotein 130 (gp130 A/T, rs1900173) and vascular endothelial growth factor (VEGF G/A, rs1570360) polymorphisms and recurrent implantation failure (RI...

full text

Vascular Endothelial Growth Factor Level in Patients With Oral Lichen Planus: A Systematic Review and Meta-Analysis Study

Background: According to the possible role of Vascular Endothelial Growth Factor (VEGF) in the incidence of oral lichen planus lesions, it seems necessary to evaluate it in patients with Oral Lichen Planus (OLP). Objective: The present study aims to investigate the level of VEGF in patients with OLP. Methods: In this systematic review and meta-analysis study, the search was conducted in online...

full text

Elevated Levels of Serum Vascular Endothelial Growth Factor-A Are Not Related to NK Cell Parameters in Recurrent IVF Failure

BACKGROUND Vascular Endothelial Growth Factor and NK cells have an interrelated role in angiogenesis that is critical for placentation and success of in vitro fertilization. An attempt was made to assess a possible relationship between the two in this study. METHODS A case control study was performed comparing the serum levels of VEGF-A and its receptor VEGF-R1 with levels of NK cells, activa...

full text

Association between the Functional Polymorphism of Vascular Endothelial Growth Factor Gene and Breast Cancer: A Meta-Analysis

The vascular endothelial growth factor (VEGF) gene single-nucleotide polymorphism involved in the regulation of the protein levels has been implicated in breast cancer. However, the published studies have produced contentious and controversial results. Herein, we performed a meta-analysis (from January to October 2013); to further evaluate the association between +936 C/T polymorphism and the r...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 27  issue 2

pages  150- 158

publication date 2020-03-01

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023