Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran

Authors

  • A. R. Jabbari Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran
  • Gh. R. Moazeni Jula 1Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran
  • M. Esmaelzadeh Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran
Abstract:

Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. multocida isolates belonged tocapsular type A. The sequence alignment of the fragment showed a high similarity (>96%) with thepublished sequences of P. multocida hya gene in the Gene Bank. It was recognised that P. multocida capsulargroup A is the dominant cause of fowl cholera in Iran.

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Journal title

volume 7  issue 3

pages  50- 55

publication date 2006-09-30

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