Phylogeny and genetic diversity of Fusarium graminearum species complex associated with Fusarium head blight of wheat in Moghan plain (Iran)

Authors

  • Kasra Sharifi Department of Plant Pathology, College of Agriculture & Natural Resources, Science & Research Branch, Islamic Azad University, Tehran, Iran
  • Mansoureh Mirabolfathy Research Prof., Department of Plant Diseases, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
  • Rasoul Zare Research Prof., Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
  • Saeed Rezaee Assistant Prof., Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:

Thirty-seven isolates of Fusarium graminearum species complexobtained from wheat heads with Fusarium head blight symptoms were selected and used for phylogenetic studies. They were collected from different localities of Moghan plain (Ardebil province, Iran). Partial sequences of translation elongation factor 1-alpha (TEF), putative reductase (RED) and UTP-ammonia ligase (URA) genes were amplified using specific primers and sequences were analyzed using Maximum Parsimony method. Almost all strains of F. graminearum species complex belonged to F. graminearum sensu stricto. The results indicated homogeneity within F. graminearum species complex. Inter-simple sequence repeat markers (ISSR) were employed to study the genetic structure of F. graminearums.s. populations collected from five localities in Moghan plain. The ISSR markers were generated by five primers and data analysed using UPGMA method with Jaccard's coefficient. Cluster analysis showed that all isolates were divided into four clades with 82% similarity level. The analysis of molecular variation indicated that most of the gene diversity (97%) was distributed within populations, whereas 3% of the variation was found among populations. Phylogenetic species identification and genetic diversity knowledge of major agent of FHB disease will be useful in defining the risk of pathogen evolution as well as benefiting disease management strategies.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

occurrence of deoxynivalenol producing isolates of fusarium graminearum species complex associated with head blight of wheat in moghan area

the severe epidemics of fusarium head blight (fhb) as a devastating disease of cereal crops has occurred on wheat in north and northwest iran in recent years. the in vitro production of deoxynivalenol (don) was qualitatively evaluated in 41 fusarium isolates collected from wheat heads associated with the scab disease, in moghan area/northwest iran. infected wheat heads were collected during 200...

full text

Identification of Fusarium species associated with Fusarium head blight of wheat in the North of Iran and phylogenetic analysis of the dominant species

In order to determine the causal agents of Fusarium head blight, during 2015–16, wheat heads with disease symptom were collected from 10 and six main wheat production areas of Golestan and Mazandaran provinces (N Iran), respectively. A total of 431 Fusarium isolates were obtained belonging to nine Fusarium species based on their morphological characteristics. Fusarium graminearum species comple...

full text

Fusarium Head Blight of Wheat

Nebraska–Lincoln cooperating with the Counties and the United States Department of Agriculture. University of Nebraska–Lincoln Extension educational programs abide with the nondiscrimination policies of the University of Nebraska–Lincoln and the United States Department of Agriculture. © 2008, The Board of Regents of the University of Nebraska on behalf of the University of Nebraska–Lincoln Ext...

full text

Genetic diversity of Fusarium graminearum sensu lato isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina.

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 4...

full text

A Simple Method for the Assessment of Fusarium Head Blight Resistance in Korean Wheat Seedlings Inoculated with Fusarium graminearum

Fusarium head blight (FHB; scab) caused mainly by Fusarium graminearum is a devastating disease of wheat and barley around the world. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON) which are a major health concern for humans and animals. The objective of this research was to develop an easy seed or seedling inoculation assay, an...

full text

Genetic Divergence and Chemotype Diversity in the Fusarium Head Blight Pathogen Fusarium poae

Fusarium head blight is a disease caused by a complex of Fusarium species. F. poae is omnipresent throughout Europe in spite of its low virulence. In this study, we assessed a geographically diverse collection of F. poae isolates for its genetic diversity using AFLP (Amplified Fragment Length Polymorphism). Furthermore, studying the mating type locus and chromosomal insertions, we identified ha...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 18  issue 1

pages  33- 49

publication date 2017-09-21

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023