Phenotypic and genotypic characterization of Candida species isolated from candideamia in Iran

Authors

  • Mohammad Ghahri Department of Biology, School of Applied Sciences, Imam Hossein University, Tehran, Iran
  • Sedigheh Beyraghi Department of Medical Technology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Shirin Sayyahfar Department of Pediatrics, Division of Pediatric Infectious Diseases, Ali Asghar Children Hospital, Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • Zohre Baseri Department of Pathology and Laboratory Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Abstract:

Background and Purpose: Candidemia is one of the most important fungal infections caused by Candida species. Infections and mortality caused by Candida species have been on a growing trend during the past two decades. The resistance of yeasts to antifungal drugs and their epidemiological issues have highlighted the importance of accurately distinguishing the yeasts at the species level. The technique applied for yeast identification should be fast enough to facilitate the imminent initiation of the appropriate therapy. Candidemia has not been studied comprehensively in Iran yet. Regarding this, the current study aimed to assess the epidemiology of candidemia at Tehran hospitals and compare the results with the previous findings. Materials and Methods: This study was conducted on 204 positive blood cultures obtained from 125 patients hospitalized in several hospitals located in Tehran, Iran, within a period of 13 months. The yeast isolation and species identification were accomplished using several phenotypic methods (i.e., production of germ tube in human serum, culture on CHROMagar Candida, and Corn meal agar containing Tween 80) and molecular methods, such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, unknown cases were subjected to PCR sequencing. These methods were then compared in terms of accuracy, sensitivity, and speed of identification. Results: According to the results, C. albicans (62.4%) was the most common isolate, followed by C. parapsilosis (n=36, 17.5%), C. glabrata (n=18, 8.8%), C. tropicalis (n=13, 6.3%), Trichosporon asahii (n=3, 1.5%), C. kefyr (n=2, 1.0%), C. lusitaniae (n=2, 1.0%), C. intermedia (n=1, 0.5%), C. guilliermondii (n=1, 0.5%), and C. krusei (n=1, 0.5%), respectively. Conclusion: As the findings indicated, the most common species causing candidemia were C. albicans, C. parapsilosis, and C. glabrata, respectively. Children less than one year old and people with cancer were at higher risk for candidemia, compared to other groups. Moreover, phenotypic and molecular methods resulted in the identification of 65.2% and 96.6% of the isolates, respectively. Consequently, PCR-RFLP could be concluded as a more favorable technique for species identification.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Genotypic Characterization of Fungal Species Isolated From Broiler Breeder Chickens, Dead-In-Shell and Hatched Chicks

To investigate the prevalence of fungal agents in local Egyptian broiler breeder chicken’s premises, tracheal and cloacal swabs from chickens, feed, and water samples were collected. The targeted breed's dead-in-shell eggs and newly hatched chick’s samples were also tested. All fungal isolates were morphologically typed and the predominant fungal species were further subjected to molecular typi...

full text

Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran

BACKGROUND Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea. OBJECTIVES This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children with diarrhea between 2007 - 2008 in Tehran, Iran. MATERIALS AND METHODS EAEC strains were examine...

full text

phenotypic and genotypic characterization of enteroaggregative escherichia coli strains isolated from diarrheic children in iran

conclusions these results indicated that eaec strains isolated from iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries. results significant percentage of local eaec strains carried the virulence plasmid genes and formed biofilms. in addition, these strain...

full text

Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum.

INTRODUCTION Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic ...

full text

Phenotypic and Genotypic Characterization of Indigenous Lactobacillus Species from

Lactobacilli are well reckoned for their technological and probiotic significance and their exercise in dairy products for trade and industry. The present study was carried out to isolate and characterize Lactobacilli from various dairy and non dairy sources of Indian origin. The isolates were subjected to phenotypic and genotypic characterizations followed by partial 16S rDNAsequencing . As ma...

full text

Study on Phenotypic and Genotypic Characterization of Bacterial Species Isolated from Decayed Fruits

Most of the methods adopted for processing of decayed fruits and degradation of wastes are not economical. In order to achieve 100% resource recovery from wastes, a novel type of waste treatment is to be followed, so that, the recovered byproducts could be used as biofertilizers. The objective of this study is identification of bacteria for studying their phenotypic and genotypic characters usi...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 4  issue 2

pages  14- 20

publication date 2018-06

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023