????? ???? ??? PCR ?? ????? ????????????? ??????????? ?? ??? ???? ??? ???????? ?????? ??? ???? ?? ??????? ?? ???????? IS6110 ? ???? ?? 38KD
author
Abstract:
This article doesn't have abstract
similar resources
Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB, and nested-rpoB PCR Cloning
Present study was aimed to examine the diagnostic utility of polymerase chain reaction (PCR) and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB). In total 80 formalin-fixed, paraffin-embedded (FFPE) samples comprising 70 samples with definite diagnosis of EPTB and 10 samples from known non- EPTB on...
full textUse of PCR Method Based on IS6110 for Detection of Mycobacterium tuberculosis Complex in Cattle
Bovine tuberculosis caused by Mycobacterium bovis is a highly infectious zoonotic disease. When transmitted to humans the disease symptoms cannot be distinguished from infection caused by Mycobacterium tuberculosis the agent of TB in humans. Transmission of the disease to humans is through direct contact with diseased animals and consumption of unpasteurized milk and milk products. The diagnost...
full textRole of IS6110-targeted PCR, culture, biochemical, clinical, and immunological criteria for diagnosis of tuberculous meningitis.
An open prospective clinical, microbiological, and molecular analysis of a national molecular diagnostic service for tuberculous meningitis (TBM) using an in-house IS6110-targeted PCR for molecular "Fastrack" diagnosis was carried out. Between April 1997 and June 1998. Consecutive cerebrospinal fluid (CSF) samples from 131 patients were assessed. Against a culture on the same sample, PCR had a ...
full textImproved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR
BACKGROUND Culture of M. bovis from diagnostic specimens is the gold standard for bovine tuberculosis diagnostics in the USA. Detection of M. bovis by PCR in tissue homogenates may provide a simple rapid method to complement bacterial culture. A significant impediment to PCR based assays on tissue homogenates is specificity since mycobacteria other than M. bovis may be associated with the tissu...
full textUse of magnetic beads for tissue DNA extraction and IS6110 Mycobacterium tuberculosis PCR.
Polymerase chain reaction (PCR) techniques are used increasingly for the diagnosis of Mycobacterium tuberculosis infection and can be used on the DNA obtained from both frozen and formalin fixed, paraffin wax embedded tissues. However, the extraction of DNA by means of the conventional phenol/chloroform method is time consuming and requires the use of potentially dangerous chemical reagents. Th...
full textAnalysis of sequence diversity among IS6110 sequence of Mycobacterium tuberculosis: possible implications for PCR based detection
The IS6110 belongs to the family of insertion sequences (IS) of the IS3 category. This insertion sequence was reported to be specific for Mycobacterium tuberculosis complex and hence is extensively exploited for laboratory detection of the agent of tuberculosis and for epidemiological investigations based on polymerase chain reaction. IS6110 is 1361-bp long and within this sequence different re...
full textMy Resources
Journal title
volume 6 issue None
pages 9- 15
publication date 2001-03
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023