Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran
Authors
Abstract:
Mirzapour S, Darvishnia M, Bazgir E, Mirzaei Najafgholi H (2020) Pathogenic variation of Didymella rabiei isolates causing Chickpea blight in three western provinces of Iran. Plant Pathology Science 9(2):1-13. DOI: 10.2982/PPS.9.2.1 Introduction: Chickpea blight, caused by Didymella rabiei, is the most limiting factor in chickpea production areas in the world, including the western provinces of Iran. The aim of this study was to investigate the pathogenic diversity of the isolates collected from western provinces of Iran (Ilam, Lorestan and Kermanshah) on differential lines of chickpea, as well as the relationship between pathogenic diversity of the isolates and their geographical origins. Materials and Methods: During the 2017-18 chickpea growing season, samples of infected plants were collected from fields in western provinces and transferred to the laboratory. Then 100 pure isolates of D. rabiei were obtained. Based on the location of collection, the isolates were divided into 20 groups. Based on the morphological characteristics, one isolate from each group was selected as a representative for greenhouse experiments. The pathogenic diversity of theses 20 isolates was assessed on eight differential lines of chickpea. The factorial experiments were performed in a completely randomized block design under greenhouse conditions. Results: The results of analysis of variance showed that there was a significant difference between differential lines and isolates at a probability level of p<0.01. Based on the response of the differential lines, the pathogenic isolates were grouped into three pathogenic groups, including pathogenic group 1 (weakly aggressive), group 2 (aggressive or moderately aggressive), and group 3 (highly aggressive). The isolates from Kermanshah and Lorestan Province were classified into Group 1 and the Ilam isolates into Groups 1, 2 and 3. The greatest severity of the disease was found in three isolates of Ilam province. The variety ILC3996 showed the highest resistance to all isolates. Conclusion: D. rabiei isolates from three western provinces of Iran are divided into three pathogenic groups: 1 (low aggressive), 2 (moderately aggressive), and 3 (highly aggressive). Highly aggressive isolates identified in Ilam province can be used in chickpea breeding programs to produce resistant cultivars to the disease.. The wild variety ILC3996 has resistant genes against these pathogenic groups and showed resistance to all of these isolates.
similar resources
The Detection and Characterization of QoI-Resistant Didymella rabiei Causing Ascochyta Blight of Chickpea in Montana
Ascochyta blight (AB) of pulse crops (chickpea, field pea, and lentils) causes yield loss in Montana, where 1.2 million acres was planted to pulses in 2016. Pyraclostrobin and azoxystrobin, quinone outside inhibitor (QoI) fungicides, have been the choice of farmers for the management of AB in pulses. However, a G143A mutation in the cytochrome b gene has been reported to confer resistance to Qo...
full textpathogenicity variation of didymella rabiei causal agent of chickpea blight from ilam and kermanshah provinces
abstract ascochyta blight caused by didymella rabiei is one of the most important diseases of chickpea in ilam and kermanshah provinces, iran. in order to determine the pathogenicity variation of didymella rabiei populations, infected plants in field crops of different regions of both provinces were sampled. one hundred isolates were isolated by chickpea seed meal dextrose agar media. these iso...
full textMating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia
The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem piece...
full textEffects of Temperature Stresses on the Resistance of Chickpea Genotypes and Aggressiveness of Didymella rabiei Isolates
Chickpea (Cicer arietinum L.) is an important food and rotation crop in many parts of the world. Cold (freezing and chilling temperatures) and Ascochyta blight (Didymella rabiei) are the major constraints in chickpea production. The effects of temperature stresses on chickpea susceptibility and pathogen aggressiveness are not well documented in the Cicer-Didymella pathosystem. Two experiments w...
full textidentification of pathotypes in populations of didymella rabiei, the causal agent of chickpea blight in ilam and kermanshah provinces
pathogenic diversity in didymella rabiei in ilam and kermanshah provinces was assessed with the objective of finding out about the existence of pathotypes. one hundred isolates from different farms of chickpea growing regions in these provinces were collected in 2007. isolates were arranged into ten different groups based upon their sampling regions. one isolate was chosen from anyone of the te...
full textA comparative study of Turkish and Israeli populations of Didymella rabiei, the ascochyta blight pathogen of chickpea
H. Ozkilinc, O. Frenkel, S. Abbo, R. Eshed, A. Sherman, D. Shtienberg*, R. Ophir and C. Can Department of Biology, University of Gaziantep, 27310, Turkey; The Levi Eshkol School of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100; Genomics Department, The Volcani Center, Agricultural Research Organization, Bet-Dagan, 50250; and Department of Plant Pathology and Weed Research, the ...
full textMy Resources
Journal title
volume 9 issue 2
pages 1- 13
publication date 2020-08
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023