P163: The Anti-Inflammatory Effects of Human Amniotic Membrane Epithelial Cells-Derived Condition Media

The human amniotic membrane known as the innermost single epithelial-covered layer provides many applications such as applicable anti-inflammatory and anti-cancer effects. These immunomodulatory effects belongs to the epithelial cells, a type of epiblast-derived fetal stem cells which currently used for regenerative medicine and transplantation. These cells are collected by author-prepared facilities and expanded in 75 cm2 cell culture flask (Biofil) in the DMEM, 12% FBS and penicillin-streptomycin antibiotic incubated in 80% humidity, 5% CO2 for 72 hours. These cell released the special macromolecules modulate the inflammatory pathways so the 2&times;105 cells were expanded in the 25 cm2 flask and incubated in the standard incubation condition. After 72 hours, the media changed and after 5 days, the cellular supernatant were collected as the conditioned media. The U937 cell line were treated with 50% condition media and standard medium (RPMI 1640, 5% GlutaMax and 10% FBS) for one week. The level of mRNA expression of IL1&alpha; and &beta; and IL 8 were evaluated in the U937 cells after 1 week treatment with conditioned media. The obtained results illustrated the significant reduction in the IL1&alpha; and &beta; and IL 8 cellular expression in the treated cells (p<0.001). The conditioned medium obtained from expanded human amniotic membrane epithelial cells has the anti-inflammatory effects based on obtained results on U937 cell line. This properties may provide the promising way in regenerative medicine.