P-86: Longer Culture of Human Blastocysts Doesn

Culture and selection of viable blastocysts: a feasible proposition for human IVF?

In human in-vitro fertilization (IVF) embryos are routinely transferred to the uterus on day 2 or day 3 of development. Resultant implantation and pregnancy rates are disappointingly low, with only approximately 10% of embryos transferred leading to a live birth. The ability to culture embryos to the blastocyst stage should help to resolve this problem by synchronizing the embryo with the femal...

[Co-culture of mouse blastocysts and their epigenetic modification].

To discuss the effect of co-culture on the quality of mouse blastocysts and their epigenetic modification. We divided mouse zygotes into three co-culture experiment groups : with granular cells (group I ), oviduct epithelium cells (group II) and oviduct tissue (group III). Meanwhile, we set up control A (cultured in vitro, only KSOM (KCl+ simplex optimized medium)) and control B (cultured in vi...

Culture of viable human blastocysts in defined sequential serum-free media.

In human in-vitro fertilization (IVF), embryos are routinely transferred to the uterus on either day 2 or day 3 of development, resulting in a 10-15% implantation rate. However, in other mammalian species, the transfer of cleavage stage embryos, which normally reside in the oviduct, to the uterus results in a significantly lower implantation rate compared with blastocysts. It is therefore propo...

In vitro culture of embryonic disc cells from porcine blastocysts.

The aim of the present study was to define the conditions of preparation and in vitro culture of embryonic discs allowing proliferation of ES-like cells. G5-6 porcine blastocysts (G0 = day of AI) were cultured in toto; in G10-11 blastocysts, trophectoderm and primitive endoderm were microsurgically removed from embryonic discs (ED) which were cultured either on plastic or on a feeder layer. Fee...

Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts

P-67: Quantitative Expression of Pluripotency Specific-Genes in Mouse Blastocysts Produced by In Vitro Fertilization

Background: The efficiency of in vitro fertilization (IVF) is still low to be developed to blastocyst stage probably because of environmental conditions. It is likely that in vitro environment can not exactly mimic in vivo environment due to differences in media, metabolic content, atmospheric composition, temperature and pH. Therefore it may affect embryo quality by changing in embryo gene exp...