P-67: Nano-Selenium Protects Cisplatin-Induced Spermatotoxicty and Chromatin Abnormality Associated with Free Radicals Toxic Stress
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Abstract:
Background: Cisplatin (CIS), an anticancer alkylating agent, induces DNA adducts and effectively cross links the DNA strands and so affects spermatozoa as a male reproductive tract toxicant. Materials and Methods: The present study investigated the cellular/biochemical mechanisms underlying possible protective effect of selenium nano-particles (Nano-Se) as an established strong antioxidant with more bioavailability and less toxicity, on reproductive toxicity of CIS by assessment of sperm characteristics, sperm DNA integrity and chromatin quality. Histopathological analysis of testis and epididymis were performed for assessment of spermatogenic disorders. To determine the role of oxidative stress (OS) in the pathogenesis of CIS gonadotoxicvity, the level of lipid peroxidation (LPO), antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and peroxynitrite (ONOO) as a marker of nitrosative stress (NS) and testosterone (T) concentration as a biomarkers of testicular function were measured in the blood and testes. Twenty-eight rats were equally divided into four groups. A single IP dose of CIS (7 mg/kg) and protective OP dose of Nano-Se (2 mg/kg/day) were administered alone or in combination. Results: The CIS-exposed rats showed a significant increase in testicular and serum LPO and peroxynitrite level, along with a significant decrease in enzymatic antioxidants levels, diminished serum testosterone concentration and abnormal histologic findings with impaired sperm quality associated with increased DNA damage and decreased chromatin quality. Coadministration of Nano-Se significantly improved the serum testosterone, sperm quality, spermatogenesis and reduced CIS induced free radicals toxic stress and sperm DNA damage. Conclusion: The current study demonstrated that Nano- Se may be useful to prevent CIS-induced gonadotoxicity through its antioxidant potential.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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