P-65: Polycystic Ovary Syndrome Induction in NMRI Mice by Testosterone Enantate

Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo-or amenorrhea, and anovulation. A common feature of PCOS is the accumulation of small subcortical follicles and increased ovarian stromal volume, causing a characteristic ultrasound image and a basis for the most commonly used designation for the syndrome. In order to thoroughly investigate polycystic ovarian syndrome there is a need for a model for PCOS. The large number of proposed animal models for PCOS is the result of difficulties in induction of all the pathological conditions similar to those seen in human PCOS and because this syndrome is probably multifactorial. In this study, we examined the effect of daily injection of testosterone enantate on PCOS induction in mouse. Materials and Methods: To induce polycystic ovary phenotype, female NMRI mice 6-8 weeks-old were injected daily with testosterone enantate 1 mg/100 g body weight dissolved in sesame oil for 1 to 4 weeks (experiment group), while control group were injected only with vehicle alone. The ovaries were fixed, and then were used for histological studies. Results: The percentage of preantral follicles in experiment groups in 1, 2, 3 and 4 weeks were respectively %62.02, %73.21, %59.23 and %47.47; and in control groups were respectively %79.83, %63.17, %94.44 and %82.61. The percentage of antral follicles in experiment groups in 1, 2, 3 and 4 weeks were respectively %3.13, %10.09, %0 and %7.43; and in control groups were respectively %20.17, %36.82, %5 and %17.39. The percentage of cystic follicles in experiment groups in 1, 2, 3 and 4 weeks were respectively %34.85, %16.69, %40.77 and %45.09; and in control groups were respectively %0, %0, %0 and %0. In experiment group, testosterone enantate treatment significantly increased the percentage of cystic follicles and decreased the percentage of preantral and antral follicles compared to control group after four-week treatment ( p<0.05). Conclusion : Testosterone enanate could induce polycystic ovary syndrome in NMRI mice.