P-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS

Authors

Abstract:

Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as density gradient centrifugation (DGC) needs further verification. In addition, considering the fact that DGC induces EPS due to capacitation and acrosome reaction, therefore, the role of MACS before DGC (MACS-DGC) and MACS after DGC (DGCMACS) were assessed. Materials and Methods: Semen samples (n = 15) were collected from infertile individuals and were washed with Ham’s buffer and divided into three separate portions. A portion of the sample was kept as controls and a second portion was used for DGC-MACS (MACS procedure was carried out after DGC). The third portion was used for MACS-DGC (DGC procedure was carried out after MACS). Percentages of sperm with normal morphology, DNA fragmentation, protamine deficiency, EPS and caspase- 3 activity were determined in each group. Results: Combination of DGC and MACS improved the sperm quality compared to when DGC and MACS were implemented independently. However, comparison of the combined procedures showed only higher efficiency to separate active caspase in the MACS-DGC group. Conclusion: In spite of recent clinical trends in the literature, which are mainly implement DGC-MACS procedure, we strongly recommend MACS-DGC procedure.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

P-83: Which Novel Sperm Selection Method Is Functional? MACS-DGC or DGC-Zeta

Background: Selection of human spermatozoa prior to assisted reproduction is currently based on motility and morphology. Recently, Zeta method has been introduced for normal sperm selection based on sperm surface charges. In addition, one of the features of apoptosis is the externalization of phosphatidylserine (PS), which are normally present on the inner leaflet of the sperm plasma membrane. ...

full text

P-24: DGC-HOST Method: An Approach for Selection of Normal Sperm during ICSI

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...

full text

P-20: Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

full text

Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...

full text

To DGC or not to DGC: oxygen guarding in the termite Zootermopsis nevadensis (Isoptera: Termopsidae).

The ability of some insects to engage in complex orchestrations of tracheal gas exchange has been well demonstrated, but its evolutionary origin remains obscure. According to a recently proposed hypothesis, insects may employ spiracular control of gas exchange to guard tissues against long-term oxidative damage by using the discontinuous gas-exchange cycle (DGC) to limit internal oxygen partial...

full text

Distributed Geospatial Computing (DGC)

DEFINITION Distributed Geospatial Computing (DGC) refers to the geospatial computing resides on multiple computers connected through computer networks. Figure 1 illustrates DGC within the C/S architecture [1]: where the geospatial computing is conducted by the geospatial components, which can communicate with each other or communicate through wrapping applications, such as web server and web br...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 3

pages  58- 58

publication date 2013-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023