P-44: Oral Gibberellic Acid Administration Impact on Testicular Histochemistry

Background: Gibberellic acid (GBA) is a naturally occurring and commercially produced plant growth regulator which may cause a variety of effects including the stimulation of seed germination in some cases. Histochemical changes in testicular tissue including carbohydrates, lipids and alkaline phosphatase enzyme changes were evaluated. Materials and Methods: Twenty seven mature male rats after one week acclimatization period were randomly divided into three groups. Group 1 served as control group and received no treatment; group 2 was controlsham group and received 1% methanol (which was career of Gibberellic acid) and finally group 3 received 10 mg/kg gibberellic acid. All administrations were with oral gavages (every other day). After sacrificing the rats on days 15, 30 and 45 of treatment period (3 rats per day per group) stained testicular slides were prepared. These stainings include Periodic Acid Shift (PAS) for carbohydrates, Sudan Black-B staining for lipids and Alkaline Phosphatase staining to evaluate alkaline phosphatase enzyme in testicular tissue. Results: In present study revealed that level of carbohydrates in three first spermatogenesis decreased in GBA group in comparison with other groups otherwise level of lipids increased. Furthermore, level of cytoplasmic alkaline phosphatase enzyme in GBA group increased in spermatogenesis cells (especially spermatocyte I cells). Conclusion: According to obtained results, acid Gibberellic agent may have metabolic negative effects in spermatogenesis cells.