P-24: Beta Defensin 126 and Its Role in Intra-Uterine Insemination Outcome
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Abstract:
Background: Human β-defensin 126 is a small cationic glycoprotein that coats the plasma membrane of sperm during epididymal transit. It provides protection for sperms from infection-causing microbes and against the female immune system. Defensin remains on the sperm until sperm become capacitated in the female reproductive tract. DEFB126 gene is located on the subtelomeric end of 20p13 in human. DEFB126 variations were found to affect the functionality of sperm cells and thus may have an important role in the success or failure of intra-uterine insemination (IUI). Therefore, this study was designed to verify the correlation of this gene mutation with the success rate of IUI. Materials and Methods: Two cytosine nucleotide deletion of DEFB126 gene in the blood of 76 Iranian men with unexplained infertility whose wives had undergone IUI were assessed. Standard PCR and Single-strand conformation polymorphism (SSCP), Tetra PCR and Sequencing were used to confirm the results. ELISA and Immunochemistry were performed for the assessment of protein expression on sperm cells. Results: Data revealed that 24.4 percent of men, whose wife showed a negative result for IUI, were homozygote for this mutation (p≤0.05) and couples with positive IUI result showed no mutation in this gene. Also, DEFB126 protein was not observed in men who were homozygote for this mutation. Conclusion: The present study suggested that this common alteration in DEFB126 could be considered as a critical factor in the success rate of IUI operation. It is concluded that men with a homozygote mutation for DEFB126 are less fertile compared to those with a wild type DEFB126 for this mutation.
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Journal title
volume 8 issue 2.5
pages 41- 41
publication date 2014-07-01
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