P-132: The Effect of Different Fetal Bovine Serum Levels on Recombinant Chinese Hamster Ovary Cells, Expressing HumanFollicle Stimulating Hormone

Background: Follicle stimulating hormone (FSH) is one of the most common and effective gonadotropin hormones used in infertility treatment. Production of FSH as a drug is affordable as long as it is done with minimal cost. FBS is the most expensive component in medium culture furthermore; it’s being controversial to cause unnecessary suffering for the unborn calf for collection their serum. In this study, the effect of different amount of FBS on the cell viability and the expression of this protein, in the CHO cell line was investigated. Materials and Methods: The FBS of cell culture medium was reduced from 10% as reference to 5, 3 and 1% gradually and was carried out for 2-3 passage for each step. The cell viability was assessed by trypan blue staining method.The morphology of the cells characterized using microscope.Total Protein concentration was measured by Bradford's technique and then SDS-PAGE and Western blotting used for detection of rFSH. Results: The results showed that, the maximum cells productivity was at 10% FBS. Increase in cell Longevity indicated at 3% FBS. Moreover, reductions of FBS to 1% lead to change in morphology of the cells. The result of SDS page and Western Blotting demonstrated reduction in the expression of FSH following the decline in the concentration of FBS. No significant difference was found in the expression of protein at 3 and 5% of FBS. Conclusion: Using 3% of FBS for FSH production is economically advantageous. This concentration is appropriate for the growth and the viability of the cells. In addition it’s good enough for production of FSH.