P-129: Prunus Cerasus Effects on Progesterone Receptor Expression in Cumulus Cells

Background: Infertility is defined as inability to achive pregnancy after one year of unprotected regular intercourse.it has been reported that about 10-15% of young couples suffer from infertility. There is evidence that antioxidants improve the fertility rate. Prunus cerasus contains strong antioxidant activity. The aim of this study is to evaluate Prunus cerasus effects on expression of progesterone receptor in cumulus cells. Expression of progesterone receptor is of essential factor for cumulus expansion. That disorder in expression of progesterone receptor can lead to infertility. Materials and Methods: In this study, 30 adult femal mice were randomly divided in to control and experiment groups. Prunus cerasus was added to the diet of female mice for 14 days. Ovulation was induced using HMG/PMSG (10 IU) and HCG (10 IU) that injected intraperitoneally then mice was dissected and ovaries were separated. Oocytes were collected and cumulus oophorus cells were isolated. Real time-polymeras chain reaction (RT-PCR) was performed to confirm the expression of progesterone receptor in cumulus cells. Results: Data revealed that Prunus cerasus increased the expression of progestron receptor in cumulus cells. Conclusion: The recent study reported different results of the effect antioxidant in fertility. in the present study, we found that prunus cerasus can increase the expression of progestron receptor in cumulus cells. As regard study on oocyt in environment rich in antioxidant is antithesis. there are similar studies regarding to the correlation between antioxidant and fertility. Antioxidants such as prunus cerasus can be used as an important medicine to improve oocyte quality and open new opportunities for treatment of infertility.