P-124: Association between Fertilization Rate and Externilization of Phosphatidyl Serine
Authors
Abstract:
Background: Externalization of PS (EPS) is considered as a sign of early apoptosis. In addition, EPS also occurs during capacitation and the acrosome reaction. During this study we aimed to evaluate the relationship between EPS and fertilization, embryo quality and pregnancy outcomes in couples who were candidates for ICSI. Materials and Methods: Semen samples (n = 43) were collected from infertile individuals ICSI candidates and assessed according to World Health Organization guidelines for semen parameters. EPS was assessed by Annexin V and propidium iodide (PI) staining. Results: There is a significant positive correlation between percentages of fertilization and annexin-positive PI-negative (An+PI-) sperm and percentage of An+ PIsperm was significantly high in individuals with fertilization rates higher than 50%. In addition, the percentage of annexin-positive PI-positive (An+PI+) sperm in semen of the partners of pregnant women significantly differed from the partners of non-pregnant women. Furthermore, a significant negative correlation between percentages of fertilization and protamine-deficient sperm was observed. Conclusion: The result of this study suggest that An+PIis a sign of capacitation and An+PI+ is a sign of apoptosis, therefore, semen samples with high percentage of An+PI-, have a higher ability to undergo capacitation and, also have a higher chance to result in successful fertilization post-ICSI.
similar resources
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Complete fractionation of brain cephalin; isolation from it of phosphatidyl serine, phosphatidyl ethanolamine, and diphosphoinositide.
Brain cephalin (1) has been the name given to a fraction of brain phosphatides characterized by its solubility in ether and insolubility in ethyl alcohol. For many years it was assumed to be a single compound with the chemical structure of diacylglycerylphosphoryl ethanolamine. Work from this laboratory has shown brain cephalin to be a mixture (2). From it were separated two pure phosphatides; ...
full textP-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate
Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...
full textP-82: Investigation of Relationship between Acrosomal Reaction Rate and Sperm DNA Quality in The Fertilization Rate of IVF Undergoing Men
Background: It is estimated that around 15% of the couple at reproductive age are infertile couples, and approximately the half of infertilities are caused by male factor. Defective spermatogenesis is the result of several different disorders, such as endocrinological disorders, disease, malnutrition, genetic defects and maybe environmental condition. Materials and Methods: In this study 60 IVF...
full textP-126: Duration of Exposure to Polyvinylpyrrolidone (PVP): The Effects on Fertilization Rate and Embryo Development
Background: Recently, it has been found that exposure of sperm to PVP may cause submicroscopic changes in sperm structure and the sperm nucleus. The PVPinduced nuclear damage may have been due to breakdown of sperm membranes. Consequently, it is probable that exposure of sperm to PVP may impair embryo development. There are apparently no detailed reports regarding the specific effects of PVP on...
full textMy Resources
Journal title
volume 7 issue 3
pages 84- 84
publication date 2013-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023