P-106: The Computer-Assisted Vacuolated Sperm Analysis and Zygote Quality

Background: Digital image processing and analysis are used for computer assisted evaluation of sperm morphology. Sperm head morphology has been identified as a characteristic that can be used to predict a male’s semen quality. On the other hand, zygote competence analysis is an importance tool to select embryo(s) for transfer. The aim of the present study is to determine zygote quality resulted from vacuolated sperms evaluated use of computer-assisted sperm analysis techniques. Materials and Methods: The first to fourth stages are the image preprocessing stage and removing of image noises, the detection and extraction of individual spermatozoa, segmenting of the spermatozoon into various region of interest such as sperm head, midpiece and tail, and classifying of Spermatozoa as normal or abnormal. Two hundred and twenty motile spermatozoa were observed, immotiled and selected for Intracytoplasmic sperm injection (ICSI). The selected sperms were assessed and divided based on the presence/ absence vacuole, and two pronucleus (2PN) scores of resulted zygotes were studied. Results: No statistically significant differences were observed between 2 groups with regard to fertilization rate. The high frequency of scores of Z1 and Z2 zygotes were observed by using of intact sperm. The Z3-1 and Z3-4 scores increased in injected oocytes with the vacuolated sperms. Conclusion: The evaluation of sperm vacuole is a prognostic tool in the prediction of ICSI success and zygote quality.