Organophosphate compounds and their biodegradation; using enzymes as an increased efficiency approach

Authors

  • Farnoosh, Gholamreza Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:

Organophosphorus compounds are widely used in pesticides, insecticides in agriculture and as nervous chemical agents. These chemicals inhibit the acetylcholinesterase enzyme activity that is responsible for the nervous impulse in organisms. This effect leads to an increase in acetylcholine level and finally neuronal complications. Many methods are used to degrade and decontaminate these compounds, such as: the use of chemicals, burial of toxins, burning and biodegradation. The chemical and physical methods are often toxic, allergic, corrosive and nonspecific and harmful for the environment and are not usable in war spaces. Biodegradation is an effective and safe method that is performed under controlled conditions for the decomposition of various constituents, including organophosphorus compounds, by biological agents. Biodegradation is performed using microbes to detoxify and decompose contaminants. These strains contain broad substrate-degrading enzymes. Although the use of natural strains as vital catalysts is an interesting method for the treatment of organophosphorus compounds, the inability of organophosphorus compounds to cross the membrane width reduces the total catalytic power, so the use of recombinant enzymes for the decomposition of organophosphorus compounds can be of great help in removing contaminants, especially in war environments. Many enzymes have been identified and used for this purpose, but most notably include: Diisopropyl-fluorophosphatase (DFPasae), Organophosphorus acid anhydrolase (OPAA) and Organophosphorus Hydrolase (OPH). In this review, in addition to describing the organophosphorus compounds and their effects, biodegradation especially by use of enzymes was considered. The understanding and mastering of this knowledge could help researchers in the use of chemical degrading enzymes, especially organophosphates, in spray and enzymatic ointments in military environments.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

a review on biodegradation of toxic organophosphate compounds

daily, organophosphorus compounds (ops) in human life, has found wide applications. although ops have biodegradability potential, they induce clinical problems in humans and other organism. different methods are used to detoxify these compounds. in the meantime, biodegradation is preferred as a compatible way to the environment since it produces less toxic compounds. enzymes capable to degrade ...

full text

Biodegradation of Xenobiotic Compounds: An Overview

The continuous accumulation of recalcitrant xenobiotic compounds into the ecosystem released from various sources caused a serious global concern. Xenobiotics compounds are carcinogenic, mutagenic, causing teratogenic effect and persist over a long period of time in the environment. Therefore there is an urgent need for the detoxification of these compounds. Biodegradation is a technique that e...

full text

detection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts

in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...

Diversity of Ligninolytic Enzymes and Their Genes in Strains of the Genus Ganoderma: Applicable for Biodegradation of Xenobiotic Compounds?

White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplore...

full text

Enhanced biodegradation of toxic organophosphate compounds using recombinant Escherichia coli with sec pathway-driven periplasmic secretion of organophosphorus hydrolase.

Although Escherichia coli can be genetically engineered to degrade environmental toxic organophosphate compounds (OPs) to nontoxic materials, a critical problem in such whole cell systems is limited substrate diffusion. The present work examined whether periplasmic expression of organophosphorus hydrolase (OPH) resulted in better whole cell enzymatic activity compared to standard cytosolic expr...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 21  issue 5

pages  468- 478

publication date 2019-11

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023