O-3: In-Situ Preservation of Caprine CaudaEpididymal Spermatozoa at -10 Degree Centigrade
Authors
Abstract:
Background: In-situ preservation of cauda epididymal spermatozoa at -10 degree centigrade with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degree centigrade. Materials and Methods: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degree centigrade,centigrade. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 0C in a water bath for 5 min. Results: Spermatozoan characters were diminished gradually and significantly (p
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Journal title
volume 4 issue 2
pages 64- 64
publication date 2010-05-01
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