Nickel Increases Chromosomal Abnormalities by Interfering with the Initiation of DNA Repair Pathways

Authors

  • Farhang Haddad Cytogenetics, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Khadije Shahrokhabadi Molecular Genetics, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
  • Mahsa Ghorbani Genetics, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Abstract:

Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet clearly understood. Methods: To find the most nickel-vulnerable time of repair mechanisms, human dermal fibroblasts (HDF) were treated with three doses of nickel before and after X-irradiation. The induced frequency of chromosomal abnormality was studied using micronucleus assay in binucleated cells. The cytotoxicity of different treatments was established using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The results revealed that nickel treatment had a synergistic effect on inducing Micronucleus frequency only when cells were treated 2hr before X-irradiation. The X-ray treatment of the cells with 5- and 10-mM nickel had a cytotoxic effect mainly when given 6hr after the irradiation. Conclusions: The results suggest that nickel can interfere with human DNA repair mechanisms only at the start of the process, while having no significant effect on the human DNA repair mechanisms when activated.  

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Disruption of maternal DNA repair increases sperm-derived chromosomal aberrations.

Male and female germ cells can transmit genetic defects that lead to pregnancy loss, infant mortality, birth defects, and genetic diseases in offspring; however, the parental origins of transmitted defects are not random, with de novo mutations and chromosomal structural aberrations transmitted predominantly by sperm. We tested the hypotheses that paternal mutagenic exposure during late spermat...

full text

Microhomology Directs Diverse DNA Break Repair Pathways and Chromosomal Translocations

Chromosomal structural change triggers carcinogenesis and the formation of other genetic diseases. The breakpoint junctions of these rearrangements often contain small overlapping sequences called "microhomology," yet the genetic pathway(s) responsible have yet to be defined. We report a simple genetic system to detect microhomology-mediated repair (MHMR) events after a DNA double-strand break ...

full text

Frequency and the Type of Chromosomal Abnormalities in Patients with Primary Amenorrhea in Northeast of Iran

full text

Association of Fetal and Parental Chromosomal Abnormalities with Congenital Anomalies

Background & Aims: Chromosome abnormalities are a major cause of miscarriage and neonatal mortality. The present study aimed to determine the association of fetal and parents chromosomal abnormalities with congenital anomalies. Methods: A cross-sectional study was performed in a tertiary referral center (Afzalipour Hospital) over 16 months period (2011-2012). The study groups consisted of 77 fe...

full text

Chromosomal abnormalities and DNA genotyping in horses

The genetic material of animals is found in structures called chromosomes, which are contained in the nucleus of a cell. Different species of animals have different numbers and structures of chromosomes. The horse has a total of 64 chromosomes or 32 pairs (one from each parent). Two of the 64 chromosomes (one pair) are called sex chromosomes because they contain genes that determine the sex of ...

full text

control of the optical properties of nanoparticles by laser fields

در این پایان نامه، درهمتنیدگی بین یک سیستم نقطه کوانتومی دوگانه(مولکول نقطه کوانتومی) و میدان مورد مطالعه قرار گرفته است. از آنتروپی ون نیومن به عنوان ابزاری برای بررسی درهمتنیدگی بین اتم و میدان استفاده شده و تاثیر پارامترهای مختلف، نظیر تونل زنی(که توسط تغییر ولتاژ ایجاد می شود)، شدت میدان و نسبت دو گسیل خودبخودی بر رفتار درجه درهمتنیدگی سیستم بررسی شده اشت.با تغییر هر یک از این پارامترها، در...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 16  issue 4

pages  0- 0

publication date 2022-10

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023