Monitoring of abundance and diversity of Vibrio sp. bacteria in whole shrimp culture period in Gwater shrimp Complex

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Abstract:

In this study abundance and composition of Vibrio spp. In water and sediment of inlet Chanel and shrimp pond during the entire crop cycle were done in Gowater shrimp complex. Samples of  source (Inlet chanel) water and pond water werecollected one day prior to filling the pond with water and thereafter at  twenty day intervals after stocking at three pre-determined sampling  locations in two ponds (pond 1 and pond 2; sampling points denoted as A, B and C in each pond) and one locations in the source water (Inlet Chanel).Total Vibrio like organisms (TVLO) wasenumerated on Vibrio-selective medium- thiosulphate citrate bile-salt sucrose agar (TCBS; HiMedia, India).Identification of Vibrio spp. was based on their morphological, and  biochemical characteristics using the Bergey’s Manual of  Determinative Bacteriology. obtained Results during the study revealed, the average total counted vibrio bacterial in rearing water were significantly higher than in source water (P < 0.05).As well as, comparisonin  abundance and composition between two ponds with each other and Inlet Chanel are significantly differentiated. Of course compositions of source water vibrios are similar betweensampling date. The Vibrio community was represented in abundance mainly by V. metschnikovii, V. fluvialis, V. mimicusrespectively. Because of pathogenic potential of these bacteria, health management of shrimp pond is very necessary.

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Journal title

volume 6  issue 3

pages  102- 114

publication date 2016-04

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