Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran

Authors

  • A. Hosseini-Chegeni Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
  • F. Faghihi Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran| Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  • G. Goudarzi Department of Medical Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran
  • M. Sharifdini Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  • M. Tavakoli Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran
  • M.K. Ghanbari Department of National Program of Zoonotic Disease, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran
  • Z. Telmadarraiy Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran| High Education Institute of Rahyan Novin Danesh (RND), Sari, Iran
Abstract:

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran

This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma marginale in sheep and dairy cattle in West-Azerbaijan province, Iran. A total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). The extracted DNA from blood cells was screened using genus-specific (Anaplasma...

full text

Molecular Detection of Bovine Leukocytic Anaplasma Species in Isfahan, Iran

Background: A. bovis and A. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. They are obligate intracellular bacteria that can infect and cause Anaplasmosis in human and animals. Therefore, this study was carried out to detect A. bovis and A. phagocytophilum in naturally infected dairy cattle in Isfahan using molecular techniques. Materials and Methods: In this study a tot...

full text

Discrimination Between Anaplasma marginale and Anaplasma ovis by PCR-RFLP

Several tick-borne diseases with different etiological agents such as rickettsia, protozoa, bacteria and viruses can infect domestic ruminants. The only common feature between these diseases is that they can all be transmitted by ticks. Of these, Anaplasma spp. can cause diseases in the livestock with high economical losses. The Giemsa staining of blood smear is the common traditional method fo...

full text

Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran

This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma marginale in sheep and dairy cattle in West-Azerbaijan province, Iran. A total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). The extracted DNA from blood cells was screened using genus-specific (Anaplasma spp.) nested...

full text

Molecular Detection of Novel Genetic Variants Associated to Anaplasma ovis among Dromedary Camels in Iran

To the best of our knowledge, little information is available regarding the presence of Anaplasma species in camels in Iran. This study sought to investigate the presence of Anaplasma species by microscopy and polymerase chain reaction (PCR) assays in 100 healthy dromedaries (Camelus dromedarius) arriving for slaughter. The microscopic examination of Giemsa-stained blood films revealed that Ana...

full text

Molecular detection and identification of Anaplasma species in sheep from Ahvaz, Iran

Ovine anaplasmosis is a tick-borne rickettsial disease, widespread in tropical and subtropical areas. In the present study, a PCR-RFLP method based on major surface protein 4 (MSP4) gene, was utilized for the detection of Anaplasma infection in 119 sheep blood samples collected from different parts of Ahvaz in the southwest of Iran. PCR identified Anaplasma infections in 87.4% (104/119) of the ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 75  issue 1

pages  39- 46

publication date 2020-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023