Molecular Cloning and Mutagenesis of Rat Glucocerebrosidase Gene
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Abstract:
Purpose: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it. Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced. Results: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing. Conclusion: The successful sequenced Gba cDNA compared with other researchers results.
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Journal title
volume 3 issue None
pages 185- 190
publication date 2005-10
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