Modulation of CD4+ T Cell Subsets by Euphorbia microciadia and Euphorbia osyridea Plant Extracts

Authors

  • Haideh Namdari Department of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
  • Maryam Izad Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Zahra Amirghofran Department of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

Background: Euphorbia plants are traditionally used in folk medicine for infections, inflammation, and cancer. Objectives: To investigate the effects of the butanolic extracts of Euphorbia micorociadia and Euphorbia osyridea on specific transcription factors and cytokines expression of T cell subsets. Methods: Activated mouse splenocytes were cultured in the presence of non-cytotoxic concentrations of the extracts. Cells were evaluated for the gene expressions of T cell transcription factors and cytokines of T helper (Th)1 [T-bet and interferon gamma (IFNγ)], Th17 [retinoic acid receptor related orphan receptor (RORγt) and interleukin (IL)-17], and T regulatory (Treg) cells [forkhead box P3(Foxp3), IL-10, and Transforming growth factor (TGF)-β] using real-time PCR. The cytokine secretions were evaluated by ELISA and Foxp3 protein expression by flow cytometry. Results: Both E. osyridea and E. microciadia extracts at 0.1 μg/ml increased T-bet expression [>1.73 relative fold change (RFC), p1195 pg/ml, p

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Journal title

volume 14  issue 2

pages  134- 150

publication date 2017-06-01

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