Measurement of Epsilon Antitoxin in Sheep Sera by ELISA

measurement of epsilon antitoxin in sheep sera by elisa

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Modified Toxin-binding Inhibition (tobi) Test for Quantification of Epsilon Antitoxin in Serum from Immunized Sheep

This study evaluated the use of the ToBI test as a substitute for the in vivo technique of the mouse serum neutralization test to manage quality control of vaccines against clostridiosis. Serum samples with known concentrations of anti-epsilon antibodies were obtained from 3 sheep immunized with vaccine against C. perfringens type D epsilon toxoid. The main results obtained indicate correlation...

Preparation of Standard Clostridium perfringens Antitoxin in the Sheep

Measurement of transcobalamin by ELISA.

BACKGROUND Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin. METHODS gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture ...

Measurement of aflatoxin M1 by ELISA method in milk samples produced by cattle in farms of Shahre Ghods, Shahriar, Iran

Background/objective: Aflatoxin M1 (AFM1) is a metabolite of the aflatoxin B1 found in the liver of livestock, as a result of feeding livestock with contaminated food. Certain species of Aspergillus are responsible for producing aflatoxin. The present study was performed to evaluate the measurement of AFM1, by ELISA method, in milk samples collected from dairy farms of Shahre Ghods, Shahriar (T...

Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)

Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...