Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation

Authors

  • Jian Xu Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
  • Peng Lun Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
  • Peng Sun Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
  • Yanxiu Chen Department of Neurology, Liaocheng People’s Hospital, Liaocheng 252000, China
  • Yihe Dou Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
  • Zeyu Wu Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
Abstract:

Objective(s): The study explored the neuroprotective role of Kalirin-7 (Kal-7) in Neuro-2A cells after oxygen-glucose deprivation and reperfusion (OGD/R) treatment.Materials and Methods: The study used an OGD/R model of mouse Neuro-2A neuroblastoma cells in vitro. Cells were transfected with pCAGGS-Kal-7 to up-regulating kal-7. Then cell proliferation and apoptosis were respectively analyzed by Trypan blue exclusion method and flow cytometry. To examine the involvement of Rac1, cells were treated with Rac1-GTP inhibitor NSC23766 before treatment with OGD/R. Expressions of Bax, Bcl-2, Rac1, and down-stream targets of Rac1 were analyzed by Western blot.Results: Kal-7 significantly decreased OGD/R induced cell apoptosis (P

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volume 21  issue 10

pages  992- 997

publication date 2018-10-01

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