INVESTIGATION OF SHV, TEM, CTX-M GENES FREQUENCY IN ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES IN CHILDREN WITH UTI IN RASHT CITY

Abstract Introduction and objective: Urinary tract infections (UTIs) is one of common infections in children which rapidly acquiring more resistance to antimicrobial drugs in the worldwide.  The aim of this study was to evaluate the frequency of SHV, TEM and CTX-M genes in Escherichia Coli and Klebsiella Pneumoniae strains isolated from UTI in children in 17 Shahrivar hospital from Rasht city. Materials & Methods: In this descriptive cross-sectional study, 49 strains of Escherichia Coli and Klebsiella Pneumoniae isolated from 17 Shahrivar hospital from Rasht city and identified by biochemical tests. The resistance and susceptibility of the strains to antibiotics was determined by Kirby Bauer and broth dilution methods. To evaluate frequency of SHV, TEM and CTX-M genes in the isolates was used PCR method. To statistical analysis, χ2 test was used with SPSS software version 19. Results: In this study, the most resistance was observed to Ceftriaxone (88.9%) and ampicillin (81.5%) in Escherichia Coli and to ampicillin (95.5%) and Ceftriaxone (77.3%) in Klebsiella Pneumoniae. Of 49 isolates, 89.8% isolates had SHV gene, 89.8% isolates had CTX-M gene, and 100% isolates had TEM gene.   Conclusion: It appears that one of the reasons of increasing multi drug resistance in nosocomial isolates of UTI in Rasht be increase of horizontal transfer of plasmid genes between these isolates.