Investigation of Enzyme Immobilization Effects on its Characteristics

Authors

  • Javad Mohammadi Department of Environmental Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan ,Iran.
  • Mazyar Peyda Department of Environmental Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan ,Iran
  • Mehdi Mohammadi National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
  • Mohammad Reza Mehrasbi Department of Environmental Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan ,Iran
Abstract:

Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve their characteristics. Methods; In this study silica coated magnetite nanoparticles were Functionalized with GPTSM as a linker, then immobilization reaction performed by using  various amounts of lipase B from Candida Antarctica (CALB), for the next step immobilization effects on thermal stability and optimum pH were investigated in comparison with free CALB. Results; Results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°C while free CALB loss its activity at the same condition. Conclusion; Immobilization of CALB on Fe3O4@SiO2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free CALB.

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Journal title

volume 1  issue None

pages  183- 188

publication date 2016-09

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