Investigation of Antimicrobial Effect of Berberine on Ciprofloxacin and Imipenem Resistance Acinetobacter baumannii Isolated from Hamadan Hospitals

Authors

  • Hassan Mahmoudi Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
  • Leili Shokoohizadeh Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  • Mohammad Yousef Alikhani Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  • Nayreh Zare Fahim Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:

Background: Acinetobacter baumannii is one of the causes of nosocomial infections, especially in the intensive care unit. The emergence of multidrug-resistant strains of A. baumannii has caused many problems. One of the ways to handle the phenomenon of antibiotic resistance is the use of herbal medicines and their derivatives in place of or in combination with antibiotics. The aim of this study was to investigate the effect of inhibitory effects of berberine as a barberry derivative on clinical isolates of A. baumannii, resistant to ciprofloxacin and imipenem in Hamadan hospitals. Materials and Methods: In this study, 70 clinical isolates of A. baumannii. were identified and diagnosed using conventional microbiology. Resistance of isolates was detected against imipenem and ciprofloxacin by disk diffusion and broth microdilution method. Minimum inhibitory concentration (MIC) of berberine as well as its combined effect with antibiotics were performed using broth microdilution method. Results: The results of this study showed that more than 90% of isolates are resistant to ciprofloxacin and imipenem. Imipenem and ciprofloxacin MICs  were dtermined from 8 to 28  and 4 to 32 μg / mL, respectively. The berberine decreased the imipenem and ciprofloxacin MIC from zero to two fold and zero to one-fold, respectively Conclusion: High level resistance to imipenem and ciprofloxacin among A.baumannii isolates is cause of concern. Berberin, in combination with imipenem and ciprofloxacin, reduces MIC to a proper level, which can be used as an effective agent to reduce antibiotic resistance in bacteria.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Antimicrobial Resistance Pattern of Acinetobacter baumannii Strains Isolated from Intensive Care Unit Patients

ABSTRACT             Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen that affects different groups of people, especially intensive care unit (ICU) patients. The prevalence of infections caused by this bacterium is very high. Today, prevalence of infections caused by multidrug-resistant (MDR) and extreme-drug resistant (XDR) strains is increasing. This study aime...

full text

Molecular Investigation of Resistance to Disinfectants in Acinetobacter Baumannii Isolates Collected From Qazvin Hospitals, Iran (2017)

Background Acinetobacter baumannii is an important agent of nosocomial infections. There are several reports on the presence of disinfectants-resistant Acinetobacter baumannii developed by qacE and qacEΔ1 genes in medical centers.  Objective The current study aimed to assess the relevant antimicrobial susceptibility and to determine the frequency of genes encoding resistance to disinfectants i...

full text

Antimicrobial Resistance Determinants in Imipenem-nonsusceptible Acinetobacter calcoaceticus-baumannii Complex Isolated in Daejeon, Korea

BACKGROUND Members of the Acinetobacter calcoaceticus-baumannii (Acb) complex are important opportunistic bacterial pathogens and present significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we investigated the integrons and various genes involved in resistance to carbapenems, aminoglycosides, and fluoroquinolones in 56 imipenem-nonsusceptible Acb ...

full text

Drug Resistance Patterns and Genotyping of Acinetobacter baumannii Strains Isolated from Patients Admitted to Shahrekord Teaching Hospitals Using REP-PCR

Background & objectives: In recent years, Acinetobacter baumannii has been shown to be associated with several nosocomial infections, including pneumonia, bacteraemia, urinary tract infections, wound infection and meningitis. This organism can survive in the hospital environment and rapidly develops resistance to many antibiotics. The molecular genotyping can increase our knowledge about the sp...

full text

Antibiotic Resistance Patterns and Molecular Typing of Acinetobacter Baumannii Strains Isolated from Burn Patients in Iran

Background and Aims: Acinetobacter baumannii (A. baumannii) is an important multidrug-resistant opportunistic pathogen frequently causing various nosocomial infections and is a serious threat to burn patients. These infections are usually caused by the outbreak strains. The aim of this study was to show antibiotic resistance pattern and molecular typing of A. baumannii genes isolates collected ...

full text

an investigation of the impact of self monitoring on langauge teachers motivational practice and its effect on learners motivation

the central purpose of this study was to conduct a case study about the role of self monitoring in teacher’s use of motivational strategies. furthermore it focused on how these strategies affected students’ motivational behavior. although many studies have been done to investigate teachers’ motivational strategies use (cheng & d?rnyei, 2007; d?rnyei & csizer, 1998; green, 2001, guilloteaux & d?...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 14  issue None

pages  44- 54

publication date 2020-03

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023