Investigation of Acid-Neutralizing Property of Bacillus cereus GUF8
Authors
Abstract:
Extreme pHs, especially acidic pHs, are among the environmental stresses that some bacteria face. The most important strategies that bacteria employ for acid adaptation include either production of acidophilic proteins, or raising the pH of the microenvironment by secretion of basic compounds including ammonia produced by the action of urease. Considering the importance of acid-neutralizing bacteria, we previously isolated Bacillus sp. GUF8 from acidic soil of a tea plantation and identified it as Bacillus cereus using standard bacterial identification methods and 16S rDNA sequencing. Tea plantation soil is intrinsically acidic and so allows survival for only acidophilic or acid-tolerant bacteria. In the light of this fact, we investigated acid tolerance and/or adaptation property of B. cereus GUF8 in the present study. Our results showed that the isolate is an acid-tolerant and not acidophilic strain which neutralizes the acidic microenvironment by secreting urease. Urease production increases until the pH reaches to about 8.0-9.0, whilst the isolate grows optimally at around pHs 6.0-7.0 and there is a lag period of about two days prior to active growth on media at pH 4.0. The results suggest that the isolate has potential for use as a neutralizing agent in acidified environments.
similar resources
Characterization of an a-Amylase with Broad Temperature Activity from an Acid-Neutralizing Bacillus cereus Strain
Bacillus sp. GUF8, isolated from acidic soil samples of a tea farm was identified as Bacillus cereus, based on 16S rDNA sequencing and standard bacterial identification methods. Following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. Consequently, thermostability and kinetic parameters of the purified enzyme wer...
full textcharacterization of an a-amylase with broad temperature activity from an acid-neutralizing bacillus cereus strain
bacillus sp. guf8, isolated from acidic soil samples of a tea farm was identified as bacillus cereus, based on 16s rdna sequencing and standard bacterial identification methods. following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. consequently, thermostability and kinetic parameters of the purified enzyme wer...
full textDipicolinic acid-less mutants of Bacillus cereus.
When J. Powell (Biochem. J. 54:210, 1953) established dipicolinic acid (DPA) as an important constituent of bacterial spores, microbiologists became concerned about the function this compound serves and about its biosynthetic pathway. It is now generally considered that DPA is involved in the heat resistance of spores, but the mechanism is unknown. A number of schemes have been proposed for its...
full textAntimicrobial Effects of Honey on Bacillus Cereus
Abstract Background and Objective: Honey is a healthy and nutritious food that has been used for a long time as a treatment for different diseases. One of the applied properties of honey is its antimicrobial effect, which differs between different types of honey due to variation of phenolic and antioxidant compositions. This study aimed to assess antimicrobial effect of honey on Bacillus cereu...
full textBacillus cereus responses to acid stress.
Coping with acid environments is one of the prerequisites for the soil saprophytic and human pathogenic lifestyle of Bacillus cereus. This minireview highlights novel insights in the responses displayed by vegetative cells and germinating spores of B. cereus upon exposure to low pH as well as organic acids, including acetic acid, lactic acid and sorbic acid. Insights regarding the possible acid...
full textinvestigation of thermal comfort properties of woven sport fabric using blend of estabragh fibers
امروزه لباس در نظر ورزشکاران و کسانی که برای اوقات فراغت خود و یا برای رسیدن به اندامی متعادل، ورزش می کنند؛ بسیار با اهمیت است. احساس مطلوب از لباس در زمره خصوصیات راحتی پوشش می باشد. خصوصیات انتقال رطوبت لباس، در ارزیابی راحتی حسی و حرارتی منسوجات تولید شده از آن ها بسیار مهم است. هدف از این تحقیق، معرفی پارچه جدید است که متشکل از الیاف استبرق با خواص منحصر به فرد می باشد. استبرق لیف طبیعی تو...
My Resources
Journal title
volume 3 issue 1
pages 18- 25
publication date 2017-07-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023