Inhibition of Foot-and-Mouth Disease Virus Replication by Hydro-alcoholic and Aqueous-Acetic Acid Extracts of Alhagi maurorum
Authors
Abstract:
Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.
similar resources
antiviral activity of alhagi maurorum medik’s methanolic extract on foot and mouth disease virus (fmdv) in cell cultures
conclusions the present investigation supports the use of the methanolic extract of a. maurorum as an antiviral drug in folk and modern medicine. this activity may justify its use as a source of natural compounds to control fmdv in infected animals. background foot and mouth disease (fmd) is an infectious disease of cloven-hoofed animals that has adverse economic impacts. given the prevalence o...
full textFoot-and-mouth Disease Virus Ribonucleic Acid
-The foot-and-mouth disease virus-RNA polymerase complex was released from membrane particulates present in the cytoplasm of infected baby hamster kidney cells. The soluble polymerase complex was fractionated by zonal centrifugation in sucrose gradients. Two polymerase complexes (RNA and protein complex) active in the cell-free system were isolated and had S-rate ranges of 20-70S and 100-300S, ...
full textNucleotide mismatches of foot-and-mouth disease virus during replication.
As there is a lack of error correction mechanisms during RNA replication, foot-and-mouth disease virus (FMDV) has a very high mismatch rate, which leads to a high mutation rate, in the range of 10(-3) to 10(-5) per nucleotide site per genome replication. We examined the nucleotide mismatch of FMDV during replication, based on the whole genomes of the 7 serotypes retrieved from NCBI. With the Me...
full textOrganic Circulation and persistence of Foot – and – Mouth disease virus type O in guinea pig
Since, the most susceptible laboratory animal to Foot–and–Mouth disease virus (FMDV) is guinea pig, and then one milliliter of FMDV type (O) concentrations of 106-106.5 TCID50was inoculated intradermally (ID) to 10 plantar surface of guinea pig (right side). Guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30...
full textMy Resources
Journal title
volume 14 issue 1
pages 85- 96
publication date 2018-01-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023