In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells

Authors

  • Fatemeh Naseri Central Laboratory, Ferdowsi University of Mashhad, Mashhad, Iran
  • Maryam Moghaddam Matin 1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
  • Masoud Fereidoni Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
  • Nasser Mahdavi Shahri Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Stem Cell Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
  • Somayeh Naderi Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:

The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared from the scaffolds after two weeks of culture with stem cells and studied by histological methods. The interactions of scaffolds with MSCs were also studied by electron microscopy. Histological and electron microscopy studies revealed human mesenchymal stem cell adhesion, migration, division and maintenance during the 14 days of culture in vitro. According to the results, scaffolds prepared from rat intestine matrix could be a suitable scaffold for studying in vitro cell behaviors such as division, migration and attachment. These various behaviors of cultured cells might be due to inductive effects of the extracellular matrix derived scaffold. However, more investigations are required to discover the exact effects of this scaffold and its interactions with mesenchymal stem cells.

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Journal title

volume 6  issue 3

pages  251- 255

publication date 2015-09-01

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