In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold

in vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold

background: extensive full-thickness burns require replacement of both epidermis and dermis. in designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. methods: in the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

In vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold.

BACKGROUND Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. METHODS In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Comprehensive analysis of zinc derivatives pro-proliferative, anti-apoptotic and antimicrobial effect on human fibroblasts and keratinocytes in a simulated, nutrient-deficient environment in-vitro

Zinc as therapeutic agent in skin and wound care has been known for centuries, but its role is controversial and comprehensive investigations in nutrient-deficient environments are lacking. We aimed to provide a broad analysis of different zinc derivatives on proliferation, apoptosis and antimicrobial properties in a simulated nutrient-deficient environment in-vitro. Human fibroblasts (CRL2522)...

A denatured collagen microfiber scaffold seeded with human fibroblasts and keratinocytes for skin grafting.

Biomaterial scaffolds are categorized into artificial or natural polymers, or combinations of the two. Artificial polymers often undergo serum protein adsorption, elicit foreign body and encapsulation immune responses post-implantation. Large pore bovine electrospun collagen I was therefore screened as a candidate for human keratinocyte and fibroblast cell scaffolds. Human HaCaT keratinocyte an...