In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were retrieved from the GenBank and a fusiongene was designed to produce a chimeric fusion protein. Secondary and tertiary structures and specificitiesof fusion protein were determined by online software. Results showed that the designed fusion gene construction is suitable for chimeric fusion protein expression.