Improvement in the yield of oral poliovirus vaccine (Sabin strains) produced in human diploid cells

improvement in the yield of oral poliovirus vaccine (sabin strains) produced in human diploid cells

0

Improvement in the yield of oral poliovirus vaccine (Sabin strains) produced in human diploid cells.

When stationary cultures of human diploid cells in conventional Roux bottles were replaced by the development of cells in roller bottles, the yield of poliovirus, Sabin strains type 1 and 2, showed an increase of 0.5 to 1 log10. No significant difference was noticed in the yield of these viruses cultured in WI-38 and MRC-5 human diploid cells.

Efficacy of oral poliovaccine made in human diploid cells

Experience with production and control of attenuated poliovirus (Subinstrains) in human diploid cells

Sabin Vaccine Reversion in the Field: a Comprehensive Analysis of Sabin-Like Poliovirus Isolates in Nigeria.

UNLABELLED To assess the dynamics of genetic reversion of live poliovirus vaccine in humans, we studied molecular evolution in Sabin-like poliovirus isolates from Nigerian acute flaccid paralysis cases obtained from routine surveillance. We employed a novel modeling approach to infer substitution and recombination rates from whole-genome sequences and information about poliovirus infection dyna...

A recombinant virus between the Sabin 1 and Sabin 3 vaccine strains of poliovirus as a possible candidate for a new type 3 poliovirus live vaccine strain.

Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region encoding mainly the viral capsid proteins had little correlation with the neurovirulence or attenuation phenotype of the virus. The results suggested that new vaccine strains of type 2 and type 3 pol...