Immunophenotypic Characterization of the Leukemic B-cells from Iranian Patients with Chronic Lymphocytic Leukemia: Association between CD38 Expression and Disease Progression

Authors

  • Ahmad Shamsian Khoramabadi Clinic of Hematology and Oncology, Firozgar Hospital, Faculty of Medicine, Iran University of Medical Sciences
  • Fazel Shokri Department of Immunology, School of Public Health, Medical Sciences /Tehran University, Tehran, Iran | National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Hojatollah Rabbani Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden | Monoclonal Antibody Research Center, Avesina Research Institute
  • Mahmood Jeddi-Tehrani Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden | Monoclonal Antibody Research Center, Avesina Research Institute
  • Mohammad Hojjat Farasangi Department of Immunology, School of Public Health, Medical Sciences /Tehran University, Tehran, Iran
  • Ramazan Ali Sharifian Clinic of Hematology and Oncology, Vali-Asr Hospital, Faculty of Medicine, Medical Sciences/ University of Tehran
  • Seyed Mohsen Razavi Clinic of Hematology and Oncology, Firozgar Hospital, Faculty of Medicine, Iran University of Medical Sciences
Abstract:

Background: Patients with B-cell chronic lymphocytic leukemia (B-CLL) have hetero-geneous clinical courses, thus several biological parameters need to be added to the cur-rent clinical staging systems to predict disease outcome. Recent immunophenotypic stud-ies performed mainly in Western populations have demonstrated the prognostic value of CD38 and ZAP-70 expression in B-CLL. Objectives: To investigate the expression pat-tern of a variety of membrane antigens on leukemic cells from Iranian patients with CLL and to find out if there are any differences in the expression of these markers between in-dolent and progressive groups. Methods: In the present study, peripheral blood samples from 87 Iranian patients with B-CLL were analysed by flow cytometry. Results: In all cases, the neoplastic cells displayed B-CLL phenotype (CD5+/CD19+/sIg+). The vast ma-jority of the cases expressed CD23, but failed to stain for CD3 or CD14. The leukemic cells of most patients expressed CD27 (84/87, 95.4%) and CD45RO (74/87, 83.9%) molecules, suggesting a memory B-cell phenotype. Comparison between the indolent (n=42) and progressive (n=37) patients revealed significantly higher frequency and inten-sity of CD38 expression in progressive group (40.5%) compared to indolent (11.9%) pa-tients (p

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Journal title

volume 5  issue 1

pages  25- 35

publication date 2008-03-01

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