Identification of aggressive species of Trichoderma from button mushroom farms (Agaricus bisporus) using morphological and molecular methods

Authors

  • M. Mehrparvar M.Sc. Student, Department of Plant Pathology, College of Agriculture, Tarbiat Modarres University, Tehran, Iran
  • Y. R. Danesh Assistant Prof., Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
  • Zh. Zargarzadeh M.Sc. Student, Department of Plant Pathology, College of Agriculture, Tarbiat Modarres University, Tehran, Iran
Abstract:

Different species of Trichoderma especially T. harzianum (Th2 biotype in Europe and Th4 in America) considered as causal agent of green mould disease on button mushroom (Agaricus bisporus) casing beds. In this study, 100 fungal isolates were collected and purified from different mushroom growing units in Tehran province and Urmia (NW Iran). Totally, 60 isolates of T. harzianum, 27 isolates of T. virens, seven isolates of T. hamatum and six isolates of T. inhamatum were morphologically identified. The main infectious agent was T. harzianum. Specific primers were used for identification of different biotypes. 

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Journal title

volume 12  issue 1

pages  83- 89

publication date 2011-08-23

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