Identification and characterization of pigmented photoreceptor-producing microorganisms using FTIR spectroscopy
Authors
Abstract:
The identification, differentiation and classification of microorganisms have been subjects of research for many years. Recently, Fourier transform infrared (FTIR) spectroscopy techniques have gained attention in the characterization and classification of microorganisms based on biochemical profiles and cell structure characteristics. In the present study, the characterization and differentiation of pigmented photoreceptor-producing microorganisms using FTIR spectroscopy was carried out. For this purpose some microorganisms were isolated from different environments, of which three photoreceptor-producing bacteria were selected to limit the scope of the study to one phenotypic characteristic. Genomic relatedness among the isolated strains was investigated and it was shown that these strains had similarities to the Kushneria marisflavi, Halobacillus halophilus and Halobacillus faecis species. In addition, Halobacterium salinarum was investigated as a typical representative photoreceptor-producing archaeon. Spectra (500-4000 cm-1) of the intact cells and crude extracted pigments were recorded on an FTIR spectrometer and compared with each other. The similarities among the spectra were evaluated using hierarchical cluster analysis and compared with the phylogenic tree based on genomic study. Our results demonstrate that hierarchical clustering based on extracted pigments shows separation of strains more distinctly than those based on intact cells. The results of the present study suggest that FTIR analysis of bacterial pigments is an easy and economical technique comparable to other phylogenetic markers, for the differentiation and characterization of bacteria.
similar resources
Isolation, Identification and Characterization of Lipase Producing Microorganisms from Environment
Objective: Lipases represent an important group of hydrolytic proteins. These enzymes find numerous applications in the industrial sector to provide regioselective molecules of commercial interest. The present investigation was carried out to isolate and identify a bacterium that can quickly produce this enzyme from very simple and cost effective nutrient medium. Materials and Methods: Lipid as...
full textCharacterization of microorganisms using UV resonance Raman spectroscopy and chemometrics.
The past decade has seen an increased interest in the application of several physicochemical analytical techniques for the rapid detection and identification of microorganisms. We report the development of UV resonance Raman (UVRR) spectroscopy for the reproducible acquisition of information rich Raman fingerprints from endospore-forming bacteria belonging to the genera Bacillus and Brevibacill...
full textCharacterization of Oil Shale by Ftir Spectroscopy
The o p t i c a l advantages and d a t a handl ing c a p a b i l i t i e s of F o u r i e r transform inf r a r e d (FT-IR) ins t ruments have l e d t o a resurgence of t h e use of i n f r a r e d spec t roscopy f o r t h e c h a r a c t e r i z a t i o n of energy r e l a t e d m a t e r i a l s . Most of t h e work t h a t has so f a r been r e p o r t e d has involved t h e c h a r a c t e ...
full textDetection and Identification of Microorganisms in Mixed Cultures by Nanoparticle-Induced Nanospr Enhanced FTIR Spectroscopy and Chemometrics
Routine identification of pathogenic microorganisms predominantly based on nutritional and biochemical tests is a time-consuming process; the delay may lead to fatal consequences at times. In this work, nanoparticle-induced nanoSPR enhanced IR spectroscopy was used in conjunction with a background elimination data processing algorithm to directly identify microorganisms in mixed cultures. It wa...
full textIsolation and FTIR spectroscopy characterization of chitin from local sources
Chitin the second most abundant after cellulose biopolymer found in nature after, is produced by many living organisms, and is present usually in a complex with other polysaccharides and proteins. Chitin was found as a major component in arthropods (insects, crustaceans, arachnids and myriapods), nematodes, algae and fungi. In this study Chitin was isolated from various species (crab, squilla, ...
full textMy Resources
Journal title
volume 7 issue 1
pages 1- 9
publication date 2017-06-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023