Human Reoviruses Serotype 3 Effectively Target Huh-7 Cells

Authors

  • Angila Ataei-Pirkooh Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Reihaneh Kazemi Hepatitis & AIDS department, Pasteur institute of Iran, Tehran, Iran.
Abstract:

Abstract: Background  and  Aims:  Huh-7  is  a  cell  line  that  was  derived  from  a  liver  tumor  of  a  Japanese  man.  Hepatocellular  carcinoma  (HCC)  is  considered  as  a  primary  liver  cancer.  Highly  resistant  tumor  to  treatment  which  causes  the  death  of  many  patients  annually.  Thus,  targeting  the  cancer  cells  by  using  a  new  method  could  be  effective  in  therapy  of  this  cancer.  Reoviruses  are  oncolytic  viruses  that  can  infect  and  kill  tumor  cells,  which  have  an  activated  Ras  signaling  pathways,  while  normal  cells  are  resistant  to  infection  and  replication  of  these  viruses.  The  aim  of  this  study  was  to  evaluate  the  effect  of  oncolytic  human  reovirus  on  Huh-7  cell  line  in  vitro.  Materials  and  Methods:  Human  reovirus  serotype  3,  Huh-7  cell  line  and  normal  human  fibroblasts  were  used  in  this  study.  After  virus  purification  and  plaque  assay,  human  reoviruses  were  inoculated  into  the  Huh-7  cells  and  human  normal  fibroblasts  as  negative  control.  Virus  cytopathic  effect,  cell  viability  and  viral  RNA  replication  were  assessed  at  the  different  time  of  post-infection. Results:  Virus  cytopathic  effects  and  cell  lysis  were  clearly  observed  and  reovirus  RNA  replication  was  detected  in  the  Huh-7  cells,  whereas  normal  human  fibroblasts  were  resistant  against  reovirus  infection. Conclusion:  The  result  of  the  present  study  showed  that  human  reoviruses  serotype  3  can  destroy  the  Huh-7  cells.  Accordingly,  the  use  of  human  reovirus  could  be  considered  as  a  potential  therapy  for  HCC  and  liver  cancer.

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Journal title

volume 14  issue None

pages  0- 0

publication date 2020-12

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