Human plasma derived drugs separation by fractionation of plasma with polyethylene glycol
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Abstract:
Background: There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. There are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consuming method and it needs machinery and plant. In the present study the fractionation of human plasma by polyethylene glycol was investigated. Objectives: The purpose of this study was to investigate the possibility of the fractionation of human plasma by polyethylene glycol. Materials and Methods: Human plasma fractionation was carried out by using polyethylene glycol at different concentrations from five to twenty percent, and it was followed by centrifugation. After each step of addition of polyethylene glycol the supernatant was removed for further fractionation by addition of higher concentration of polyethylene glycol. Results: Suitable intermediate sources for protein purification were obtained by fractionation of human plasma by polyethylene glycol. Fibrinogen in fraction 5% , IgG and IgM in fraction 10%, IgA in fraction 20%, and finally albumin and α1-Antitrypsin in supernatant 20% of polyethylene glycol were achieved. Conclusion: By our study we could obtain four different fractions as intermediate sources for protein purification which cannot be easily obtained from plasma fractionation by cold ethanol fractionation.
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human plasma derived drugs separation by fractionation of plasma with polyethylene glycol
background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...
full textHuman Plasma Derived Drugs Separation by Fractionation of Plasma with Polyethylene Glycol
Human blood as a unique tissue (1), contains many important proteins with therapeutic uses (2). As a biological drugs, virus inactivation is one of the crucial steps in their preparations of these plasma derived proteins (3-6). The most important proteins in human plasma are: albumin, immunoglobulin, fibrinogen, and coagulation factors VII, VIII and IX (7). There are varieties of purification t...
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Journal title
volume 12 issue 3
pages 82- 85
publication date 2014-10-01
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