Human Fibroblast Switches to Anaerobic Metabolic Pathway in Response to Serum Starvation: A Mimic of Warburg Effect

Authors

  • Amrollah Mostafazadeh Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
  • Arian Hajian Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
  • Haleh Akhavan Niaki Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
  • Hamid Reza Khorasani Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
  • Monireh Golpour Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
  • Roya Mehrasa Blood Transfusion Research Center, High Institute For Research And Education In Transfusion Medicine, Tehran, Iran.
Abstract:

Fibroblasts could be considered as connective tissue cells that are morphologically heterogeneous with diverse functions depending on their location and activity. These cells play critical role in health and disease such as cancer and wound by Production of collagen, fibronectin, cytokines and growth factors. Absence of insulin and other growth factors in serum deprivation condition and similarity of this condition to the environment of tumor cells and ulcer made us to investigate anaerobic glycolysis in these cells. To this end, we cultured fibroblasts isolated from fresh human newborn foreskin in serum free medium for 16, 24, 48 and 72 hrs and measured glucose consumption, lactate secretion and intracellular LDH in these cells. The results showed despite the lack of insulin, the 16hr serum starved fibroblasts consumed glucose similar to non-starved fibroblasts control. Moreover, in this condition these cells secreted higher levels of lactate and exhibited higher levels of intracellular LDH in comparison to non-starved fibroblasts control. Thus it could be concluded that in serum starvation condition, the newborn human dermal fibroblasts may change the metabolic strategy to Warburg effect. This finding opens a new perspective to further understanding the basic mechanisms involved in communication between tumor cells and fibroblasts.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

human fibroblast switches to anaerobic metabolic pathway in response to serum starvation: a mimic of warburg effect

fibroblasts could be considered as connective tissue cells that are morphologically heterogeneous with diverse functions depending on their location and activity. these cells play critical role in health and disease such as cancer and wound by production of collagen, fibronectin, cytokines and growth factors. absence of insulin and other growth factors in serum deprivation condition and similar...

full text

from linguistics to literature: a linguistic approach to the study of linguistic deviations in the turkish divan of shahriar

chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...

15 صفحه اول

Metabolic response to human growth hormone during prolonged starvation.

The metabolic response to human growth hormone (HGH) was studied in five obese subjects in the fed state and during prolonged (5-6 wk) starvation. In the fed state (three subjects), HGH induced an elevation in basal serum insulin concentration, a minimal increase in blood and urine ketone levels, and a marked reduction in urinary nitrogen and potassium excretion resulting in positive nitrogen a...

full text

a frame semantic approach to the study of translating cultural scripts in salingers franny and zooey

the frame semantic theory is a nascent approach in the area of translation studies which goes beyond the linguistic barriers and helps us to incorporate cognitive and cultural factors to the study of translation. based on rojos analytical model (2002b), which centered in the frames or knowledge structures activated in the text, the present research explores the various translation problems that...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 3  issue None

pages  74- 80

publication date 2014-02

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023