HPV18 E7 induces the over-transcription of eIF4E gene in cervical cancer

Authors

  • Haixian Kang Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
  • Min Gao Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China |People's Hospital of Dongguan, No. 3 South of Wandao Road, Dongguan, Guangdong Province 523059, PR China
  • Sen Wang Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
  • Tianyun Pang Obstetrics and Gynecology Department of the first Affiliated Hospital of Guangdong Medical College, No. 57 Avenue of the people, Zhanjiang, Guangdong Province 524023, PR China|Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
  • Xinrong Hu Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China|Pathology Department of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
  • Yi Zhao Cancer Institute of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
  • Yunhong Yao Pathology Department of Guangdong Medical College, No. 1 Xincheng Road, Dongguan, Guangdong Province 523808, PR China
Abstract:

Objective(s):Eukaryotic translation initiation factor 4E (eIF4E) is overexpressed in cervical cancer (CC). However, the molecular mechanisms are unclear. This study aimed to investigate the molecular mechanism of eIF4E gene overexpression in CC. Materials andMethods:The human papillomavirus (HPV) type 18 E7 and eIF4E mRNAs were measured following knock down or overexpression of E7 gene by RT-PCR and real-time PCR. Cell counting kit-8 assay was used to determine the cell proliferation. Flow cytometry was used to analyze the cell cycle and apoptosis. Transwell system was employed to determine the cell migration. Results:Overexpression of E7 gene increased eIF4E mRNA level by 24.3% (P

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volume 18  issue 7

pages  684- 690

publication date 2015-07-01

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