HLA-DRB1, DQA1 and DQB1 Alleles and Hap-lotypes Frequencies in Iranian Healthy Adult Responders and Non-Responders to Recombi-nant Hepatitis B Vaccine

Authors

  • Ali Akbar Amirzargar National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran | Deartment of Immunology, Faculty of Medicine, Medical Sciences/University of Tehran, Tehran, Iran
  • Amir Amanzadeh National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Fazel Shokri National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran | Department of Immunology, School of Public Health, Medical Sciences/University of Tehran, Tehran, Iran
  • Manijeh Yousefi Behzadi National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Mohammad Ali Shokrgozar National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Nahid Ahmadi National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Nilufar Mohseni National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Zohreh Arjang National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Abstract:

Background: Different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis B (HB) vaccine do not produce protective levels of anti-HB antibody, a phenomenon which could be linked to certain human leukocyte an-tigen (HLA) class-II alleles or haplotypes. Objectives: The present study was under-taken to determine the frequency of HLA class-II alleles in Iranian healthy adult re-sponders and non-responders to HB vaccine. Methods: Twelve non-responders (anti-HBs antibody100 IU/L) were tissue typed for HLA class-II. HLA-DRB1, DQB1 and DQA1 alleles were determined using polymerase chain reaction based on sequence specific primers (PCR-SSP) technique. Accessibility to excess amount of genomic DNA was possible using Epstein-Barr virus (EBV)-transformed B-cells established from all vaccinees. Results: Our results demon-strated increased frequencies of HLA- DRB1*07, DRB1*03, DRB1*04, DQB1*0201, DQA1*0201 alleles and HLA- DRB1*07/DQB1*0201/DQA1*0201 and DRB1*04/DQB1*0302/DQA1*03011 haplotypes in the non-responder group. Com-parison between responders and non-responders revealed only a significant difference for DQB1*0201 allele (p

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hla-drb1, dqa1 and dqb1 alleles and hap-lotypes frequencies in iranian healthy adult responders and non-responders to recombi-nant hepatitis b vaccine

background: different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis b (hb) vaccine do not produce protective levels of anti-hb antibody, a phenomenon which could be linked to certain human leukocyte an-tigen (hla) class-ii alleles or haplotypes. objectives: the present study was under-taken to determine the frequency of hla class-ii alleles in ...

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HLA-DRB1, DQA1 and DQB1 alleles and haplotypes frequencies in Iranian healthy adult responders and non-responders to recombinant hepatitis B vaccine.

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Journal title

volume 5  issue 2

pages  92- 99

publication date 2008-06-01

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